Single cell protein and oil production from solid cocoa fatty acid distillates co-fed ethanol

Single cell protein and oil production from solid cocoa fatty acid distillates co-fed ethanol

Bioresource Technology

10.1016/j.biortech.2023.129630

http://dx.doi.org/10.1016/j.biortech.2023.129630

Eric Charles Peterson, Rowanne Siao, Gi Gi Chua, Coleen Toledo Busran, Renata Pavlovic, Aaron Thong, Christian Hermansen, Naazneen Sofeo, Yoganathan Kanagasundaram, Melanie Weingarten, Nic Lindley

General Medicine, Bioengineering, Sustainability and the Environment, Environmental Engineering, Waste Management and Disposal, renewable energy

05 August 2023

Peterson, E. C., Siao, R., Chua, G. G., Busran, C. T., Pavlovic, R., Thong, A., Hermansen, C., Sofeo, N., Kanagasundaram, Y., Weingarten, M., & Lindley, N. (2023). Single cell protein and oil production from solid cocoa fatty acid distillates co-fed ethanol. Bioresource Technology, 387, 129630. https://doi.org/10.1016/j.biortech.2023.129630

The use of solid lipid sidestreams have been overlooked as a feedstock for the production of microbial biomass for food and feed applications. Oleaginous yeasts can convert waste lipids to microbial oils, yet little to no recent work has examined the utilization of solid fatty acid distillates (FADs), which are a significant residue from vegetable oil processing. Yarrowia lipolytica and Rhodosporidium toruloides cultivated on cocoa fatty acid distillates (CFAD) generated final cell dry weight values >40 g/L, strong productivity (3.3 g/Lh) and showed rich protein (>45%) and lipid content (>25%). Interestingly, microbial oils were >65% unsaturated fatty acids, compared <20% unsaturated content in FAD. Importantly, to overcome mass-transfer limitations associated with bioconversion of this solid residue, for the first time ethanol was applied as a co-substrate to solubilize FAD residues while acting as a co-substrate. Here, FAD residues from cocoa deodorization have been demonstrated to be high energy feedstocks that represent an attractive substrate for the production of both single cell protein and oil (SCPO).

Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0)

This research / project is supported by the A*STAR - 2021 Horizontal Technology Coordinating Office Seed Fund
Grant Reference no. : C211018002

This research / project is supported by the A*STAR - HHP Domain Industry Alignment Fund Pre-Positioning (IAF-PP)
Grant Reference no. : H20H6a0028

https://oar.a-star.edu.sg/communities-collections/articles/19286

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Singapore Institute of Food and Biotechnology Innovation