CRISPR–Cas9 strategy for activation of silent Streptomyces biosynthetic gene clusters

CRISPR–Cas9 strategy for activation of silent Streptomyces biosynthetic gene clusters
Title:
CRISPR–Cas9 strategy for activation of silent Streptomyces biosynthetic gene clusters
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Nature Chemical Biology
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Publication Date:
10 April 2017
Citation:
Zhang, M., Wong, F., Wang, Y. et al. CRISPR–Cas9 strategy for activation of silent Streptomyces biosynthetic gene clusters. Nat Chem Biol 13, 607–609 (2017). https://doi.org/10.1038/nchembio.2341
Abstract:
Here we report an efficient CRISPR–Cas9 knock-in strategy to activate silent biosynthetic gene clusters (BGCs) in streptomycetes. We applied this one-step strategy to activate multiple BGCs of different classes in five Streptomyces species and triggered the production of unique metabolites, including a novel pentangular type II polyketide in Streptomyces viridochromogenes. This potentially scalable strategy complements existing activation approaches and facilitates discovery efforts to uncover new compounds with interesting bioactivities.
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Funding Info:
Funded by National Institutes of Health (GM077596) (H.Z.), the A*STAR Visiting Investigator Program (H.Z.), and the National Research Foundation, Singapore (NRF2013-THE001-094) (M.M.Z., F.T.W., Y.H.L., E.L.A. and H.Z.). NMR data collection at the UIUC IGB Core was funded by NIH (S10-RR028833).
Description:
This is a post-peer-review, pre-copyedit version of an article published in Nature Chemical Biology. The final authenticated version is available online at: https://doi.org/10.1038/nchembio.2341; Article also available for free on PubMed Central® (PMC);
ISSN:
1552-4450
1552-4469
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