Li, Y., Wong, Y. L., Lee, M. Y., Li, Q., Wang, Q.-Y., Lescar, J., Shi, P.-Y. and Kang, C. (2016), Secondary Structure and Membrane Topology of the Full-Length Dengue Virus NS4B in Micelles. Angew. Chem.. doi: 10.1002/ange.201606609
Dengue virus nonstructural protein 4B (NS4B) is a membrane protein consisting of 248 residues with a crucial role in virus replication and interference with the host innate immunity. The dengue virus serotype 3 NS4B was reconstituted into lyso-myristoyl phosphatidylglycerol (LMPG) micelles. Backbone resonance assignment of NS4B was obtained using conventional solution NMR experiments. Further studies suggested that NS4B contained eleven helices and four of them are potential transmembrane regions. This study provides atomic level information for an important drug target to control flavivirus infections.
A*STAR BMRC IAF grant (111105) A*STAR JCO grant (1431AFG102/1331A028). NMRC/CBRG/0073/2014 from MOH. A*STAR Biospecialties project (1526004161).