Colour Metrology for Colourimetric Holographic Biosensor Application

Colour Metrology for Colourimetric Holographic Biosensor Application

Measurement Science and Technology

10.1088/1361-6501/adf767

https://doi.org/10.1088/1361-6501/adf767

Jing Zhang, Yuanjie Liu, Zixuan Li, Leon Cong Zhi Chan, Shengkai Yu

color metrology, HUE, holographic, Biosensor

14 August 2025

Zhang, J., Liu, Y., Li, Z., Chan, C. Z., & Yu, S. (2025). Colour Metrology for Colourimetric Holographic Biosensor Application. Measurement Science and Technology. https://doi.org/10.1088/1361-6501/adf767

A novel methodology is developed to identify colour information of the holographic pH sensor using modern smartphones by leveraging its integrated LED light source and CCD camera. This study addressed the difference in colour appearance of the same sample inspected with different devices. The colour metrology solution includes standardizing the lighting and perceiving condition, calibrating reference colour standards under standard colour measurement conditions, and calibrating the holographic sensor’s colour appearance taken by smartphone device by using calibrated reference colour standards. Results after calibration demonstrated a significant improvement in consistency, differences in HUE of biosensor captured by different smartphones can be improved by up to 50%. This confirms that the smartphone, as a measuring tool, coupled with this metrology solution is able to accurately and reliably measure the colour of holographic biosensors, offering a more consistent colourimetric analysis.

Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0)

There was no specific funding for the research done

This is the Accepted Manuscript version of an article accepted for publication in Measurement Science and Technology. IOP Publishing Ltd is not responsible for any errors or omissions in this version of the manuscript or any version derived from it. The Version of Record is available online at https://doi.org/10.1088/1361-6501/adf767.

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