Induction of Human T-cell and Cytokine Responses Following Vaccination with a Novel Influenza Vaccine

Induction of Human T-cell and Cytokine Responses Following Vaccination with a Novel Influenza Vaccine
Title:
Induction of Human T-cell and Cytokine Responses Following Vaccination with a Novel Influenza Vaccine
Other Titles:
Scientific Reports
Keywords:
Publication Date:
20 December 2018
Citation:
Skibinski, D.A.G., Jones, L.A., Zhu, Y.O. et al. Induction of Human T-cell and Cytokine Responses Following Vaccination with a Novel Influenza Vaccine. Sci Rep 8, 18007 (2018). https://doi.org/10.1038/s41598-018-36703-7
Abstract:
Cell mediated immunity plays a vital role in defense against influenza infection in humans. Less is known about the role of vaccine-induced cell mediated immunity and the cytokine responses elicited. We measured CD4+ and CD8+ T-cell reactivity in human subjects following vaccination with licensed trivalent influenza vaccine and a novel virus-like particle based vaccine. We detected influenza-specific CD4+ T-cell responses following vaccination with the licensed trivalent influenza vaccine and found that these correlated with antibody measurements. Administration of the novel virus-like particle based vaccine elicited influenza-specific CD4+ and CD8+ T-cell responses and the induction of the cytokines IFN-γ, IL-17A, IL17F, IL-5, IL-13, IL-9, IL-10 and IL-21. Pre-existing cytokine responses influenced the profile of the cytokine response elicited by vaccination. In a subset of individuals the VLP vaccine changed pre-vaccination production of type 2 cytokines such as IL-5 and IL-13 to a post-vaccination type 1 cytokine signature characterized by IFN-γ. A transcriptional signature to vaccination was found to correlate with antibody titer, IFN-γ production by T-cells and expression of a putative RNA helicase, DDX17, on the surface of immune cells.
License type:
http://creativecommons.org/licenses/by/4.0/
Funding Info:
The work was supported by the A*Star Program in Translational Research on Infectious Disease Bio Medical Research Council (BMRC/EDB IAF311003 to J.E.C.).
Description:
ISSN:
2045-2322
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