Liu, D., Chen, S., & Win Naing, M. (2020). A review of manufacturing capabilities of cell spheroid generation technologies and future development. Biotechnology and Bioengineering, 118(2), 542–554. doi:10.1002/bit.27620
Abstract:
Spheroid culture provides cells with a three-dimensional environment that can better mimic physiological conditions compared to monolayer culture. Technologies involved in the generation of cell spheroids are continuously being innovated to produce spheroids with enhanced properties. In this paper, we review the manufacturing capabilities of current cell spheroid generation technologies. We propose that spheroid generation technologies should enable tight and robust process controls to produce spheroids of consistent and repeatable quality. Future technology development for the generation of cell spheroids should look into improvement in process control, standardization, scalability and monitoring, in addition to advanced methods of spheroid transfer and characterization.
License type:
Publisher Copyright
Funding Info:
This study is supported by Bioprocessing Technology Institute (BTI) under the Agency for Science, Technology and Research of Singapore.
Description:
This is the peer reviewed version of the following article: Liu, D., Chen, S., & Win Naing, M. (2020). A review of manufacturing capabilities of cell spheroid generation technologies and future development. Biotechnology and Bioengineering, 118(2), 542–554. doi:10.1002/bit.27620, which has been published in final form at http://dx.doi.org/10.1002/bit.27620. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Use of Self-Archived Versions.