Bioprocessing Technology Institute

Publication date Article title Author(s)
18 May 2021 Semi-Automated Glycoproteomic Data Analysis of LC-MS Data Using GlycopeptideGraphMS in Process Development of Monoclonal Antibody Biologics Kuin Tian Pang, Shi Jie Tay, Corrine Wan, Ian Walsh, Matthew S. F. Choo, Yuan Sheng Yang, Andre Choo, Ying Swan Ho, Terry Nguyen-Khuong
7 May 2021 Bruton's tyrosine kinase phosphorylates DDX41 and activates its binding of dsDNA and STING to initiate type 1 interferon response Koon-Guan Lee, Susana Soo-Yeon Kim, Lin Kui, Dominic Chih-Cheng Voon, Marjorie Mauduit, Pradeep Bist, Xuezhi Bi, Natasha Ann Pereira, Chengcheng Liu, Bindu Sukumaran, Laurent Renia, Yoshiaki Ito, Kong-Peng Lam
7 May 2021 Production of Highly Sialylated Recombinant Glycoproteins Using Ricinus communis Agglutinin-I-Resistant CHO Glycosylation Mutants John S. Y. Goh, Kah Fai Chan, Zhiwei Song
26 Feb 2021 Multiomics analyses of cytokines, genes, miRNA, and regulatory networks in human mesenchymal stem cells expanded in stirred microcarrier-spinner cultures Alan Tin-Lun Lam, Alison P. Lee, Premkumar Jayaraman, Kah Yong Tan, Deepak Raghothaman, Hsueh Lee Lim, He Cheng, Lihan Zhou, Andy Hee-Meng Tan, Shaul Reuveny, Steve Oh
30 Jan 2021 A new and simplified anion exchange chromatographic process for the purification of cell-grown influenza A H1N1 virus Ta Duy Tien, Chu Kai Ling, Bte Soonaan Nur Izzati, Chin Christine, Ng Say Kong, Zhang Wei
27 Jan 2021 A SH3_5 Cell Anchoring Domain for Non-recombinant Surface Display on Lactic Acid Bacteria Pei Kun Richie Tay, Pei Yu Lim, Dave Siak-Wei Ow
13 Jan 2021 Multi-pronged approach to human mesenchymal stromal cells senescence quantification with a focus on label-free methods Weichao Zhai, Jerome Tan, Tobias Russell, Sixun Chen, Dennis McGonagle, May Win Naing, Derrick Yong, Elena Jones
11 Dec 2020 A Scalable Suspension Platform for Generating High-Density Cultures of Universal Red Blood Cells from Human Induced Pluripotent Stem Cells Jaichandran Sivalingam, Yu SuE, Zhong Ri Lim, Alan T.L. Lam, Alison P. Lee, Hsueh Lee Lim, Hong Yu Chen, Hong Kee Tan, Tushar Warrier, Jing Wen Hang, Nazmi B. Nazir, Andy H.M. Tan, Laurent Renia, Yuin Han Loh, Shaul Reuveny, Benoit Malleret, Steve K.W. Oh
20 Nov 2020 TACI constrains TH17 pathogenicity and protects against gut inflammation Sean Jing Xiang Tan, Arleen Sanny, Xijun Ou, Sze-Wai Ng, Alex Xing Fah Wong, Gloria Hoi Wan Tso, Biyan Zhang, Pei-Yun Teo, Susana Soo-Yeon Kim, Alison P. Lee, Shengli Xu, Kong-Peng Lam, Andy Hee-Meng Tan
9 Nov 2020 Utility of Ion-Mobility Spectrometry for Deducing Branching of Multiply Charged Glycans and Glycopeptides in a High-Throughput Positive ion LC-FLR-IMS-MS Workflow Edward G. Pallister, Matthew S. F. Choo, Ian Walsh, Jien Nee Tai, Shi Jie Tay, Yuan Sheng Yang, Say Kong Ng, Pauline M. Rudd, Sabine L. Flitsch, Terry Nguyen-Khuong
4 Nov 2020 A review of manufacturing capabilities of cell spheroid generation technologies and future development Dan Liu, Sixun Chen, May Win Naing
29 Sep 2020 Emerging Roles of Downstream of Kinase 3 in Cell Signaling Jia Tong Loh, Kay Hui Teo Joey, Hong-Hwa Lim, Kong-Peng Lam
27 Aug 2020 Clustering and Curation of Electropherograms: An Efficient Method for Analysing Large Cohorts of Glycomic Profiles in Tracking the Effects of Multivariate Parameters in Bioprocessing Operations Walsh Ian, Choo Matthew S F, Chiin Sim Lyn, Mak Amelia, Tay Shi Jie, Rudd Pauline M, Yuansheng Yang, Choo Andre, Ying Swan Ho, Nguyen-Khuong Terry
11 Aug 2020 A comprehensive CHO SWATH-MS spectral library for robust quantitative profiling of 10,000 proteins Kae Hwan Sim, Lillian Chia-Yi Liu, Hwee Tong Tan, Kelly Tan, Daniel Ng, Wei Zhang, Yuansheng Yang, Stephen Tate, Xuezhi Bi
1 Mar 2020 ASK1 mediates Nur77 expression in T-cell receptor mediated thymocyte apoptosis Jianxin Huo, Shengli Xu, Kong-Peng Lam
20 Feb 2020 Glycolysis and Oxidative Phosphorylation Play Critical Roles in Natural Killer Cell Receptor-Mediated Natural Killer Cell Functions Zixi Wang, Di Guan, Shu Wang, Louis Yi Ann Chai, Shengli Xu, Kong-Peng Lam
18 Feb 2020 Human mesenchymal stem cell therapy for cartilage repair: Review on isolation, expansion, and constructs Alan T. L. Lam, Shaul Reuveny, Steve Kah-Weng Oh
25 Jan 2020 Investigation of the effect of salt additives in Protein L affinity chromatography for the purification of tandem single-chain variable fragment bispecific antibodies Serene W. Chen, Darryl Tan, Yuan Sheng Yang, Wei Zhang
7 Jan 2020 Preclinical Activity of Embryonic Annexin A2-Specific Chimeric Antigen Receptor T Cells Against Ovarian Cancer Leonard Leong, Heng Liang Tan, Simeon Cua, Kylie Su Mei Yong, Qingfeng Chen, Andre Choo
15 Nov 2019 Opportunities for Antibody Discovery Using Human Pluripotent Stem Cells: Conservation of Oncofetal Targets Heng Liang Tan, Andre Choo
14 Nov 2019 A human expression system based on HEK293 for the stable production of recombinant erythropoietin Christine Lin Chin, Justin Bryan Goh, Harini Srinivasan, Kaiwen Ivy Liu, Ali Gowher, Raghuvaran Shanmugam, Hsueh Lee Lim, Matthew Choo, Wen Qin Tang, Andy Hee-Meng Tan, Terry Nguyen-Khuong, Meng How Tan, Say Kong Ng
7 Aug 2019 In vivo surveillance and elimination of teratoma‐forming human embryonic stem cells with monoclonal antibody 2448 targeting annexin A2 Heng Liang Tan, Bao Zhu Tan, Winfred Xi Tai Goh, Simeon Cua, Andre Choo
4 Jul 2019 von Hippel-Lindau Protein Maintains Metabolic Balance to Regulate the Survival of Naive B Lymphocytes Shengli Xu, Jianxin Huo, Yuhan Huang, Melissa Aw, Shuwen Chen, Shiya Mak, Lian Yee Yip, Ying Swan Ho, Sze Wai Ng, Andy Hee-Meng Tan, Alison Lee, Xijun Ou, Kong-Peng Lam
20 Jun 2019 Rapid Detection of Senescent Mesenchymal Stromal Cells by a Fluorescent Probe Joshur Ang, Yong‐An Lee, Deepak Raghothaman, Premkumar Jayaraman, Kim L. Teo, Fahima J. Khan, Shaul Reuveny, Young‐Tae Chang, Nam‐Young Kang, Steve Oh
10 Jun 2019 Dok3–protein phosphatase 1 interaction attenuates Card9 signaling and neutrophil-dependent antifungal immunity Jia Tong Loh, Shengli Xu, Jian Xin Huo, Susana Soo-Yeon Kim, Yue Wang, Kong-Peng Lam
9 Jun 2019 Combining Glucose Units, m/z, and Collision Cross Section Values: Multiattribute Data for Increased Accuracy in Automated Glycosphingolipid Glycan Identifications and Its Application in Triple Negative Breast Cancer Katherine Wongtrakul-Kish, Ian Walsh, Lyn Chiin Sim, Amelia Mak, Brian Liau, Vanessa Ding, Noor Hayati, Han Wang, Andre Choo, Pauline M. Rudd, Terry Nguyen-Khuong
4 Jun 2019 Attenuated glutamine synthetase as a selection marker in CHO cells to efficiently isolate highly productive stable cells for the production of antibodies and other biologics Pao-Chun Lin, Kah Fai Chan, Irene A. Kiess, Joselyn Tan, Wahyu Shahreel, Sze-Yue Wong, Zhiwei Song
4 Jun 2019 FAIM: An Antagonist of Fas-Killing and Beyond Jianxin Huo, Shengli Xu, Kong-Peng Lam
21 May 2019 Characterization of a GDP-Fucose Transporter and a Fucosyltransferase Involved in the Fucosylation of Glycoproteins in the Diatom Phaeodactylum tricornutum Zhiwei Song, Azeddine Driouich, Patrice Lerouge, Muriel Bardor, Peiqing Zhang, Carole Burel, Carole Plasson, Marie-Christine Kiefer-Meyer, Clément Ovide, Bruno Gügi, Corrine Wan, Gavin Teo, Amelia Mak
13 May 2019 GlycopeptideGraphMS: Improved Glycopeptide Detection and Identification by Exploiting Graph Theoretical Patterns in Mass and Retention Time Matthew S. Choo, Corrine Wan, Pauline M. Rudd, Terry Nguyen- Khuong
9 May 2019 Bioprocessing and biological challenges to large-scale red blood cell production from pluripotent stem cells: important factors to consider Jaichandran Sivalingam, Steve Kah-Weng Oh
8 Apr 2019 Sub-confluent culture of human mesenchymal stromal cells on biodegradable polycaprolactone microcarriers enhances bone healing of rat calvarial defect Alan Tin-Lun Lam, Eileen Jia-Hui Sim, Asha Shekaran, Jian Li, Kim-Leng Teo, Julian L. Goggi, Shaul Reuveny, William R. Birch, Steve Kah-Weng Oh
25 Feb 2019 The stress granule protein G3BP1 binds viral dsRNA and RIG-I to enhance IFN-β response Susana Soo-Yeon Kim, Lynette Sze, Kong-Peng Lam
8 Feb 2019 HEXIM1 Peptide Exhibits Antimicrobial Activity Against Antibiotic Resistant Bacteria Through Guidance of Cell Penetrating Peptide Pooi Leng Ho, Han Kee Ong, Jeanette Teo, Dave Siak-Wei Ow, Sheng-Hao Chao
1 Feb 2019 Brewing with malted barley or raw barley: what makes the difference in the processes? Yee Jiun Kok, Lijuan Ye, Jeroen Muller, Dave Siak-Wei Ow, Xuezhi Bi
13 Nov 2018 Ezh2 Controls Skin Tolerance through Distinct Mechanisms in Different Subsets of Skin Dendritic Cells Jia Tong Loh, Thomas Jun Feng Lim, Kyoko Ikumi, Takuma Matoba, Baptiste Janela, Merry Gunawan, Tatsuya Toyama, Maegan Bunjamin, Lai Guan Ng, Michael Poidinger, Akimichi Morita, Florent Ginhoux, Sayuri Yamazaki, Kong-Peng Lam, I-Hsin Su
29 Sep 2018 Review: In vitro generation of red blood cells for transfusion medicine: Progress, prospects and challenges Esmond Lee, Jaichandran Sivalingam, Zhong Ri Lim, Gloryn Chia, Low Gin Shi, Mackenna Roberts, Yuin-Han Loh, Shaul Reuveny, Steve Kah Weng Oh
7 Aug 2018 GlycanAnalyzer: Software for Automated Interpretation of N-Glycan Profiles after Exoglycosidase Digestions Ian Walsh, Terry Nguyen-Khuong, Katherine Wongtrakul-Kish, Shi Jie Tay, Daniel Chew, Tasha Jose, Christopher H. Taron, Pauline M. Rudd
2 Aug 2018 Conservation of oncofetal antigens on human embryonic stem cells enables discovery of monoclonal antibodies against cancer Heng Liang Tan, Charlene Yong, Bao Zhu Tan, Wey Jia Fong, Jayanthi Padmanabhan, Angela Chin, Vanessa Ding, Ally Lau, Lu Zheng, Xuezhi Bi, Yuansheng Yang, Andre Choo
1 Aug 2018 Meticulous optimization of cardiomyocyte yields in a 3-stage continuous integrated agitation bioprocess Sherwin Ting, Alan Lam, Gerine Tong, Allen Chen, Heming Wei, Jianjun Wu, Yue Ning Lam, Shaul Reuveny, Steve Oh
1 Jul 2018 Improved erythroid differentiation of multiple human pluripotent stem cell lines in microcarrier culture by modulation of Wnt/β-Catenin signaling Jaichandran Sivalingam, Hong Yu Chen, Bin-Xia Yang, Zhong Ri Lim, Alan Tin Lun Lam, Tsung Liang Woo, Allen Kuan-Liang Chen, Shaul Reuveny, Yuin-Han Loh, Steve Kah-Weng Oh
7 May 2018 The “less-is-more” in therapeutic antibodies: Afucosylated anti-cancer antibodies with enhanced antibody-dependent cellular cytotoxicity Natasha A. Pereira, Kah Fai Chan, Pao Chun Lin, Zhiwei Song
26 Apr 2018 Unraveling the Inconsistencies of Cardiac Differentiation Efficiency Induced by the GSK3β Inhibitor CHIR99021 in Human Pluripotent Stem Cells Filip Laco, Tsung Liang Woo, Qixing Zhong, Radoslaw Szmyd, Sherwin Ting, Fahima Jaleel Khan, Christina L. L. Chai, Shaul Reuveny, Allen Chen, Steve Oh
20 Apr 2018 GlycoStore: a database of retention properties for glycan analysis Sophie Zhao, Ian Walsh, Jodie L. Abrahams, Louise Royle, Nguyen-Khuong Terry, Daniel Spencer, Daryl L. Fernandes, Nicolle H. Packer, Rudd Pauline M., Matthew P. Campbell
19 Mar 2018 Genome-scale model-driven strain design for dicarboxylic acid production in Yarrowia lipolytica Pranjul Mishra, Na-Rae Lee, Meiyappan Lakshmanan, Minsuk Kim, Byung-Gee Kim, Dong-Yup Lee
17 Mar 2018 Proteomic Profiling of Barley Spent Grains Guides Enzymatic Solubilization of the Remaining Proteins Xuezhi Bi, Lijuan Ye, Ally Lau, Yee Jiun Kok, Lu Zheng, Daniel Ng, Kelly Tan, Dave Ow, Edwin Ananta, Christina Vafiadi, Jeroen Muller
15 Mar 2018 Kinetic Modeling of Mammalian Cell Culture Bioprocessing: The Quest to Advance Biomanufacturing Sarantos Kyriakopoulos, Kok Siong Ang, Meiyappan Lakshmanan, Zhuangrong Huang, Seongkyu Yoon, Rudiyanto Gunawan, Dong-Yup Lee
6 Mar 2018 Application of maltose as energy source in protein-free CHO-K1 culture to improve the production of recombinant monoclonal antibody Dawn Sow Zong Leong, Brian Kah Hui Teo, Janice Gek Ling Tan, Hayati Kamari, Yuan Sheng Yang, Peiqing Zhang, Say Kong Ng
2 Mar 2018 Transcription factor YY1 is essential for iNKT cell development Xijun Ou, Jianxin Huo, Yuhan Huang, Yan-Feng Li, Shengli Xu, Kong-Peng Lam
31 Jan 2018 Cadmium nanoparticles citrullinate cytokeratins within lung epithelial cells: cadmium as a potential cause of citrullination in chronic obstructive pulmonary disease Judith Müller, Joseph E McCarthy, Yurii K Gun’ko, Navin Kumar Verma, Adriele Prina-Mello, Yuri Volkov, David Hutchinson, Xuezhi Bi, Luisana Di Cristo, Laura Kickham, Dania Movia