Screening for a Potent Antibacterial Peptide to Treat Mupirocin- Resistant MRSA Skin Infections

Screening for a Potent Antibacterial Peptide to Treat Mupirocin- Resistant MRSA Skin Infections
Title:
Screening for a Potent Antibacterial Peptide to Treat Mupirocin- Resistant MRSA Skin Infections
Other Titles:
International Journal of Peptide Research and Therapeutics
Publication Date:
04 February 2017
Citation:
Ng, S.M.S., Ching, H.S.V., Xu, G. et al. Int J Pept Res Ther (2017). doi:10.1007/s10989-017-9580-x
Abstract:
Mupirocin is the first-line topical antibacterial drug for treating skin infections caused primarily by meticillin-resistant Staphylococcus aureus (MRSA). Its widespread use since its introduction more than 30 years ago has resulted in the global emergence of mupirocin-resistant strains of MRSA. Antimicrobial peptides (AMPs) are a promising class of antibacterial compounds that can potentially be developed to replace mupirocin due to their rapid membrane-targeting bactericidal mode of action and predicted low propensity for resistance development. Herein, we conducted and compared the antibacterial activities of 61 AMPs between 3 to 11 residues in length reported in the literature over the past decade against mupirocin-resistant MRSA. The most potent AMP, 11-residue peptide 50, was selected and tested against a panel of clinical isolates followed by a time-kill and a human dermal keratinocyte cytotoxicity assay. Lastly, peptide 50 was formulated into a topical spray which showed strong in vitro bactericidal effects against mupirocin-resistant MRSA. Our results strongly suggest that peptide 50 has the potential to be further developed into a new class of topical antibacterial agent for treating drug-resistant MRSA skin infections.
License type:
PublisherCopyrights
Funding Info:
A*STAR Biomedical Research Council
Description:
ISSN:
1573-3149
1573-3904
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