PICLS with human cells is the first high throughput screening method for identifying novel compounds that extend lifespan

PICLS with human cells is the first high throughput screening method for identifying novel compounds that extend lifespan

Biology Direct

10.1186/s13062-024-00455-4

https://doi.org/10.1186/s13062-024-00455-4

Mohammad Alfatah, Yizhong Zhang, Arshia Naaz, Trishia Yi Ning Cheng, Frank Eisenhaber

Chronological lifespan, anti aging

23 January 2024

Alfatah, M., Zhang, Y., Naaz, A., Cheng, T. Y. N., & Eisenhaber, F. (2024). PICLS with human cells is the first high throughput screening method for identifying novel compounds that extend lifespan. Biology Direct, 19(1). https://doi.org/10.1186/s13062-024-00455-4

Gerontology research on anti-aging interventions with drugs could be an answer to age-related diseases, aiming at closing the gap between lifespan and healthspan. Here, we present two methods for assaying chronological lifespan in human cells: (1) a version of the classical outgrowth assay with quantitative assessment of surviving cells and (2) a version of the PICLS method (propidium iodide fluorescent-based measurement of cell death). Both methods are fast, simple to conduct, cost-effective, produce quantitative data for further analysis and can be used with diverse human cell lines. Whereas the first method is ideal for validation and testing the post-intervention reproductive potential of surviving cells, the second method has true high-throughput screening potential. The new technologies were validated with known anti-aging compounds (2,5-anhydro-d-mannitol and rapamycin). Using the high-throughput screening method, we screened a library of 162 chemical entities and identified three compounds that extend the longevity of human cells.

Attribution 4.0 International (CC BY 4.0)

This research / project is supported by the Agency for Science, Technology and Research - Career Development Fund
Grant Reference no. : C210112008

This research / project is supported by the US NAM Healthy Longevity Catalyst Awards Grant - NA
Grant Reference no. : MOH-000758-00

This research / project is supported by the National Medical Research Council - Young Individual Research Grant
Grant Reference no. : MOH-001348-00

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