Ikk2 regulates cytokinesis during vertebrate development

Ikk2 regulates cytokinesis during vertebrate development
Ikk2 regulates cytokinesis during vertebrate development
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Scientific Reports
Publication Date:
14 August 2017
Shen, H., Shin, E.M., Lee, S. et al. Ikk2 regulates cytokinesis during vertebrate development. Sci Rep 7, 8094 (2017). https://doi.org/10.1038/s41598-017-06904-7
NFκB signaling has a pivotal role in regulation of development, innate immunity, and inflammation. Ikk2 is one of the two critical kinases that regulate the NFκB signaling pathway. While the role of Ikk2 in immunity, inflammation and oncogenesis has received attention, an understanding of the role of Ikk2 in vertebrate development has been compounded by the embryonic lethality seen in mice lacking Ikk2. We find that despite abnormal angiogenesis in IKK2 zygotic mutants of zebrafish, the maternal activity of Ikk2 supports embryogenesis and maturation of fertile animals and allows to study the role of IKK2 in development. Maternal-zygotic ikk2 mutants represent the first vertebrates globally devoid of maternal and zygotic Ikk2 activity. They are defective in cell proliferation as evidenced by abnormal cytokinesis, nuclear enlargement and syncytialisation of a significant portion of blastoderm. We further document that reduced phosphorylation of Aurora A by Ikk2 could underlie the basis of these defects in cell division.
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We are thankful to Drs S. Fong, C.-M. Wen, H.-L. Ang involved in preliminary morpholino-based experiments; Drs M. Balasubramanian, S. Oliferenko, P. Kaldis, E. Manser for fruitful discussions; Drs E. Manser, T. Carney, S. Rastegar and U. Straehle for reagents; the personnel of the IMCB Fish Facility for fish maintenance; the personnel of the Advanced Molecular Pathology Laboratory (IMCB) for histological service.
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