Cognate base-pair selectivity of hydrophobic unnatural bases in DNA ligation by T4 DNA ligase

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Cognate base-pair selectivity of hydrophobic unnatural bases in DNA ligation by T4 DNA ligase
Title:
Cognate base-pair selectivity of hydrophobic unnatural bases in DNA ligation by T4 DNA ligase
Other Titles:
Biopolymers
Publication Date:
06 November 2020
Citation:
Kimoto, M, Soh, SHG, Tan, HP, Okamoto, I, Hirao, I. Cognate base-pair selectivity of hydrophobic unnatural bases in DNA ligation by T4 DNA ligase. Biopolymers. 2021; 112:e23407. https://doi.org/10.1002/bip.23407
Abstract:
We present cognate base pair selectivity in template-dependent ligation by T4 DNA ligase using a hydrophobic unnatural base pair (UBP), Ds-Pa. T4 DNA ligase efficiently recognizes the Ds-Pa pairing at the conjugation position, and Ds excludes the noncognate pairings with the natural bases. Our results indicate that the hydrophobic base pairing is allowed in enzymatic ligation with higher cognate base-pair selectivity, relative to the hydrogen-bond interactions between pairing bases. The efficient ligation using Ds-Pa can be employed in recombinant DNA technology using genetic alphabet expansion, toward the creation of semisynthetic organisms containing UBPs.
License type:
Attribution 4.0 International (CC BY 4.0)
Funding Info:
This research / project is supported by the Agency for Science, Technology and Research - RIE2020 Advanced Manufacturing and Engineering Programmatic Grant scheme
Grant Reference no. : A18A9b0060

This research / project is supported by the National Research Foundation, Singapore - Competitive Research Programme
Grant Reference no. : NRF-CRP17-2017-07
Description:
ISSN:
0006-3525
1097-0282