2C-BioID: An Advanced Two Component BioID System for Precision Mapping of Protein Interactomes.

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2C-BioID: An Advanced Two Component BioID System for Precision Mapping of Protein Interactomes.
Title:
2C-BioID: An Advanced Two Component BioID System for Precision Mapping of Protein Interactomes.
Journal Title:
iScience
Keywords:
Publication Date:
15 November 2018
Citation:
2C-BioID: An Advanced Two Component BioID System for Precision Mapping of Protein Interactomes Chojnowski, Alexandre et al. iScience, Volume 10, 40 - 52
Abstract:
The modulation of protein-protein interactions (PPIs) is an essential regulatory activity defining diverse cell functions in development and disease. BioID is an unbiased proximity-dependent biotinylation method making use of a biotin-protein ligase fused to a protein of interest and has become an important tool for mapping of PPIs within cellular contexts. We devised an advanced method, 2C-BioID, in which the biotin-protein ligase is kept separate from the protein of interest, until the two are induced to associate by the addition of a dimerizing agent. As proof of principle, we compared the interactomes of lamina-associated polypeptide 2β (LAP2β) with those of lamins A and C, using 2C- and conventional BioID. 2C-BioID greatly enhanced data robustness by facilitating the in silico elimination of non-specific interactors as well as overcoming the problems associated with aberrant protein localization. 2C-BioID therefore significantly strengthens the specificity and reliability of BioID-based interactome analysis, by the more stringent exclusion of false-positives and more efficient intracellular targeting.
License type:
http://creativecommons.org/licenses/by-nc-nd/4.0/
Funding Info:
A*STAR Biomedical Research Council
Description:
ISSN:
2589-0042
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