Gates ZP, Hartrampf N. Flow-based SPPS for protein synthesis: A perspective. Peptide Science. 2020;112:e24198. https://doi.org/10.1002/pep2.24198
Abstract:
Flow‐based approaches to solid phase peptide synthesis (SPPS) have been pursued since the method's early days, with anticipated gains in speed, reaction monitoring, and ease of automation. Here, we discuss how these advantages are being realized by synthesis at elevated temperature, facilitated by a 'preheat/activation' loop. This important modification both accelerates peptide synthesis—providing a wealth of new data from in‐line monitoring—and in conjunction with an optimized protocol, extends the length of peptides routinely accessible by stepwise synthesis. Streamlined synthesis of longer peptides will address a major challenge in chemical protein synthesis: preparation of peptide segments for use in chemical ligation. The context of recent results in flow‐based SPPS is discussed, highlighting remaining challenges and future opportunities.
License type:
Publisher Copyright
Funding Info:
This research / project is supported by the A*STAR - HHP industry alignment fund pre-positioning program
Grant Reference no. : H17/01/a0/010
Description:
This is the peer reviewed version of the following article: Gates ZP, Hartrampf N. Flow-based SPPS for protein synthesis: A perspective. Peptide Science. 2020;112:e24198. https://doi.org/10.1002/pep2.24198, which has been published in final form at https://doi.org/10.1002/pep2.24198. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Use of Self-Archived Versions. This article may not be enhanced, enriched or otherwise transformed into a derivative work, without express permission from Wiley or by statutory rights under applicable legislation. Copyright notices must not be removed, obscured or modified. The article must be linked to Wiley’s version of record on Wiley Online Library and any embedding, framing or otherwise making available the article or pages thereof by third parties from platforms, services and websites other than Wiley Online Library must be prohibited.