A Novel Crosslinking Approach for Biomanufacturing of a Collagen‐Based Skin Dermal Template

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A Novel Crosslinking Approach for Biomanufacturing of a Collagen‐Based Skin Dermal Template
Title:
A Novel Crosslinking Approach for Biomanufacturing of a Collagen‐Based Skin Dermal Template
Journal Title:
Macromolecular Bioscience
Publication Date:
23 December 2024
Citation:
Chan, W. W., Roy, K. R., Le, B. Q., Ezhilarasu, H., Zhang, X., Lim, R. Y. D., Banerjee, A., Kuriakose, M., Ng, K. J., Murugan, P., Goh, C. T., Zhou, W., Naing, M. W., Raghavan, S., & Choudhury, D. (2024). A Novel Crosslinking Approach for Biomanufacturing of a Collagen‐Based Skin Dermal Template. Macromolecular Bioscience. Portico. https://doi.org/10.1002/mabi.202400457
Abstract:
Third‐degree burns result in extensive damage to the skin's epidermal and dermal layers, with limited treatment options available. Currently, xenogeneic collagen‐based skin grafts are used as scaffolds to integrate into the wound bed and provide a template for neodermis formation. Existing commercial products like Integra dermal templates rely on a time‐consuming and variable dehydrothermal (DHT) crosslinking process. This study presents a novel crosslinking process for collagen sponges, utilizing UV irradiation followed by glutaraldehyde (GA) crosslinking. This UV method allows to fine‐tune the template's crosslink density and degradation profile while significantly reducing the total crosslinking time from 48 to 24 h compared to DHT/GA crosslinking. In vitro characterization and in vivo validation are conducted using a full‐thickness skin wound mouse model. The collagen template supports the human dermal fibroblast cell line WS‐1 proliferation more effectively than the Integra template after 2 weeks in culture. Additionally, in vivo data indicate a similar level of regeneration of full‐thickness skin wounds in mouse models between the sponge and Integra templates. Furthermore, the sponge template does not elicit any abnormal angiogenic or immune responses. The crosslinking approach offers a promising alternative production process for collagen sponge scaffolds.
License type:
Publisher Copyright
Funding Info:
This research / project is supported by the Agency for Science, Technology, and Research (A*STAR) - Advanced Manufacturing and Engineering (AME) Programmatic Grant - Additive Manufacturing for Biological Materials (AMBM)
Grant Reference no. : A18A8b0059
Description:
This is the peer reviewed version of the following article: Chan, W. W., Roy, K. R., Le, B. Q., Ezhilarasu, H., Zhang, X., Lim, R. Y. D., Banerjee, A., Kuriakose, M., Ng, K. J., Murugan, P., Goh, C. T., Zhou, W., Naing, M. W., Raghavan, S., & Choudhury, D. (2024). A Novel Crosslinking Approach for Biomanufacturing of a Collagen‐Based Skin Dermal Template. Macromolecular Bioscience. Portico. https://doi.org/10.1002/mabi.202400457, which has been published in final form at https://doi.org/10.1002/mabi.202400457. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Use of Self-Archived Versions. This article may not be enhanced, enriched or otherwise transformed into a derivative work, without express permission from Wiley or by statutory rights under applicable legislation. Copyright notices must not be removed, obscured or modified. The article must be linked to Wiley’s version of record on Wiley Online Library and any embedding, framing or otherwise making available the article or pages thereof by third parties from platforms, services and websites other than Wiley Online Library must be prohibited.
ISSN:
1616-5187
1616-5195
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