Ser, Z., Gascoigne, N. R. J., MacAry, P. A., & Sobota, R. M. (2024). Protocol for structural modeling of antibody to human leukocyte antigen interaction using discovery and targeted cross-linking mass spectrometry. STAR Protocols, 5(1), 102927. https://doi.org/10.1016/j.xpro.2024.102927
Abstract:
Cross-linking mass spectrometry (XL-MS) provides low-resolution structural information to model protein structures. Here, we present a protocol to identify cross-links of purified antibody binding to purified human leukocyte antigen (HLA). We describe steps for using a discovery-based XL-MS approach followed by a targeted XL-MS approach. We then detail procedures for using the identified cross-links with other structural data for molecular docking of the antibody to HLA. This protocol has applications for modeling the interacting structure of purified antibody to antigen.
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Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0)
Funding Info:
This research / project is supported by the Agency for Science, Technology and Research - Career Development Award
Grant Reference no. : 212D800074
This research / project is supported by the National Research Fund - NRF-SIS (Shared Infrastructure Support)
Grant Reference no. : NRF2017_SISFP08
This research / project is supported by the Agency for Science, Technology and Research - Young Achiever Award
Grant Reference no. :
This research / project is supported by the Ministry of Health National Medical Research Council - NMRC grant
Grant Reference no. : MOH-000438
This research is supported by core funding from: Institute of Molecular and Cell Biology
Grant Reference no. :