Targeted ablation of Lgr5-expressing intestinal stem cells in diphtheria toxin receptor-based mouse and organoid models

Page view(s)
1
Checked on Jun 30, 2022
Targeted ablation of Lgr5-expressing intestinal stem cells in diphtheria toxin receptor-based mouse and organoid models
Title:
Targeted ablation of Lgr5-expressing intestinal stem cells in diphtheria toxin receptor-based mouse and organoid models
Other Titles:
STAR Protocols
Publication Date:
18 May 2022
Citation:
Lim, H. Y. G., Yada, S., & Barker, N. (2022). Targeted ablation of Lgr5-expressing intestinal stem cells in diphtheria toxin receptor-based mouse and organoid models. STAR Protocols, 3(2), 101411. https://doi.org/10.1016/j.xpro.2022.101411
Abstract:
Intestinal cells marked by Lgr5 function as tissue-resident stem cells that sustain the homeostatic replenishment of the epithelium. By incorporating a diphtheria toxin receptor (DTR) cassette linked to the Lgr5 coding region, native Lgr5-expressing cells are susceptible to ablation upon DT administration in vivo. A similar strategy can be used for Lgr5-expressing cells within organoids established from DTR models. Together, these in vivo and in vitro approaches will facilitate dissection of the roles of Lgr5-expressing cells residing in different tissue compartments.
License type:
Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0)
Funding Info:
This research / project is supported by the National Medical Research Council - Open Fund - Individual Research Grant
Grant Reference no. : OFIRG19may-0069

This research / project is supported by the National Research Foundation - Investigatorship
Grant Reference no. : NRF12017-03
Description:
ISSN:
2666-1667
Files uploaded:

Files uploaded:

File Size Format Action
1-s20-s266616672200291x-main.pdf 3.14 MB PDF Open