Lim, H. Y. G., Yada, S., & Barker, N. (2022). Targeted ablation of Lgr5-expressing intestinal stem cells in diphtheria toxin receptor-based mouse and organoid models. STAR Protocols, 3(2), 101411. https://doi.org/10.1016/j.xpro.2022.101411
Intestinal cells marked by Lgr5 function as tissue-resident stem cells that sustain the homeostatic replenishment of the epithelium. By incorporating a diphtheria toxin receptor (DTR) cassette linked to the Lgr5 coding region, native Lgr5-expressing cells are susceptible to ablation upon DT administration in vivo. A similar strategy can be used for Lgr5-expressing cells within organoids established from DTR models. Together, these in vivo and in vitro approaches will facilitate dissection of the roles of Lgr5-expressing cells residing in different tissue compartments.
Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0)
This research / project is supported by the National Medical Research Council - Open Fund - Individual Research Grant
Grant Reference no. : OFIRG19may-0069
This research / project is supported by the National Research Foundation - Investigatorship
Grant Reference no. : NRF12017-03