A genome-scale CRISPR screen reveals factors regulating Wnt-dependent renewal of mouse gastric epithelial cells

Page view(s)
14
Checked on May 20, 2024
A genome-scale CRISPR screen reveals factors regulating Wnt-dependent renewal of mouse gastric epithelial cells
Title:
A genome-scale CRISPR screen reveals factors regulating Wnt-dependent renewal of mouse gastric epithelial cells
Journal Title:
Proceedings of the National Academy of Sciences
Keywords:
Publication Date:
26 January 2021
Citation:
PNAS January 26, 2021 118 (4) e2016806118; https://doi.org/10.1073/pnas.2016806118
Abstract:
An ability to safely harness the powerful regenerative potential of adult stem cells for clinical applications is critically dependent on a comprehensive understanding of the underlying mechanisms regulating their activity. Epithelial organoid cultures accurately recapitulate many features of in vivo stem cell-driven epithelial renewal, providing an excellent ex vivo platform for interrogation of key regulatory mechanisms. Here, we employed a genome-scale clustered, regularly interspaced, short palindromic repeats (CRISPR) knockout (KO) screening assay using mouse gastric epithelial organoids to identify modulators of Wnt-driven stem cell-dependent epithelial renewal in the gastric mucosa. In addition to known Wnt pathway regulators, such as Apc, we found that KO of Alk, Bclaf3, or Prkra supports the Wnt independent self-renewal of gastric epithelial cells ex vivo. In adult mice, expression of these factors is predominantly restricted to non-Lgr5–expressing stem cell zones above the gland base, implicating a critical role for these factors in suppressing self-renewal or promoting differentiation of gastric epithelia. Notably, we found that Alk inhibits Wnt signaling by phosphorylating the tyrosine of Gsk3β, while Bclaf3 and Prkra suppress regenerating islet-derived (Reg) genes by regulating the expression of epithelial interleukins. Therefore, Alk, Bclaf3, and Prkra may suppress stemness/proliferation and function as novel regulators of gastric epithelial differentiation.
License type:
http://creativecommons.org/licenses/by-nc-nd/4.0/
Funding Info:
Agency for Science, Technology and Research (A*STAR); the National Research Foundation, Prime Minister’s Office, 510 Singapore under its Investigatorship Program (Award NRF-NRF12017-03); and Japan Society for the Promotion of Science Grant 17H01399.
Description:
ISSN:
0027-8424
1091-6490
Files uploaded:
File Size Format Action
There are no attached files.