Ho, N.R.Y., Lim, G.S., Sundah, N.R. et al. Visual and modular detection of pathogen nucleic acids with enzyme–DNA molecular complexes. Nat Commun 9, 3238 (2018). https://doi.org/10.1038/s41467-018-05733-0
Abstract:
Rapid, visual detection of pathogen nucleic acids has broad applications in infection management. Here we present a modular detection platform, termed enzyme-assisted nanocomplexes for visual identification of nucleic acids (enVision). The system consists of an integrated circuit of enzyme–DNA nanostructures, which function as independent recognition and signaling elements, for direct and versatile detection of pathogen nucleic acids from infected cells. The built-in enzymatic cascades produce a rapid color readout for the naked eye; the assay is thus fast (
License type:
http://creativecommons.org/licenses/by/4.0/
Funding Info:
This work was supported in part by funding from MOE (R-722-000-005-133), NMRC (NMRC/TCR/016-NNI/2016), A*STAR IMCB Independent Fellowship and NUS Early Career Research Award.