Critical role for cold shock protein YB-1 in cytokinesis

Critical role for cold shock protein YB-1 in cytokinesis

Cancers

10.3390/cancers12092473

https://doi.org/10.3390/cancers12092473

Antony Braithwaite, Sunali Mehta, Michael Algie, Tariq Al-Jabry, Cushla McKinney, Srinivasaraghavan Kannan, Chandra S Verma, Weini Ma, Jessie Zhang, Tara K Bartolec, V. Pragathi Masamsetti, Kim Parker, Luke Henderson, Maree L Gould, Puja Bhatia, Rhodri Harfoot, Megan Chircop, Torsten Kleffmann, Scott B Cohen, Adele G Woolley, Anthony J Cesare

YB-1, cold shock protein, cytokinesis, post-translational modification, phosphorylation, live-cell imaging, confocal microscopy, atomistic modelling

01 September 2020

Mehta, S.; Algie, M.; Al-Jabry, T.; McKinney, C.; Kannan, S.; Verma, C.S.; Ma, W.; Zhang, J.; Bartolec, T.K.; Masamsetti, V.P.; Parker, K.; Henderson, L.; Gould, M.L.; Bhatia, P.; Harfoot, R.; Chircop, M.; Kleffmann, T.; Cohen, S.B.; Woolley, A.G.; Cesare, A.J.; Braithwaite, A. Critical Role for Cold Shock Protein YB-1 in Cytokinesis. Cancers 2020, 12, 2473.

High levels of the cold shock protein Y-box-binding protein-1, YB-1, are tightly correlated with increased cell proliferation and progression. However, the precise mechanism by which YB-1 regulates proliferation is unknown. Here, we found that YB-1 depletion in several cancer cell lines and in immortalized fibroblasts resulted in cytokinesis failure and consequent multinucleation. Rescue experiments indicated that YB-1 was required for completion of cytokinesis. Using confocal imaging we found that YB-1 was essential for orchestrating the spatio-temporal distribution of the microtubules, β-actin and the chromosome passenger complex (CPC) to define the cleavage plane. We show that phosphorylation at six serine residues was essential for cytokinesis, of which novel sites were identified using mass spectrometry. Using atomistic modelling we show how phosphorylation at multiple sites alters YB-1 conformation, allowing it to interact with protein partners. Our results establish phosphorylated YB-1 as a critical regulator of cytokinesis, defining precisely how YB-1 regulates cell division.

http://creativecommons.org/licenses/by/4.0/

This research is supported by core funding from the Bioinformatics Institute, ARES.

The full paper is available for download at the publisher's URL: https://doi.org/10.3390/cancers12092473

https://oar.a-star.edu.sg/communities-collections/articles/17080

2072-6694

Bioinformatics Institute