Genetic alphabet expansion technology by creating unnatural base pairs.

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Genetic alphabet expansion technology by creating unnatural base pairs.
Title:
Genetic alphabet expansion technology by creating unnatural base pairs.
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Chemical Society Reviews
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Publication Date:
07 November 2020
Citation:
@Article{D0CS00457J, author ="Kimoto, Michiko and Hirao, Ichiro", title ="Genetic alphabet expansion technology by creating unnatural base pairs", journal ="Chem. Soc. Rev.", year ="2020", volume ="49", issue ="21", pages ="7602-7626", publisher ="The Royal Society of Chemistry", doi ="10.1039/D0CS00457J", url ="http://dx.doi.org/10.1039/D0CS00457J", abstract ="Recent advancements in the creation of artificial extra base pairs (unnatural base pairs{,} UBPs) are opening the door to a new research area{,} xenobiology{,} and genetic alphabet expansion technologies. UBPs that function as third base pairs in replication{,} transcription{,} and/or translation enable the site-specific incorporation of novel components into DNA{,} RNA{,} and proteins. Here{,} we describe the UBPs developed by three research teams and their application in PCR-based diagnostics{,} high-affinity DNA aptamer generation{,} site-specific labeling of RNAs{,} semi-synthetic organism creation{,} and unnatural-amino-acid-containing protein synthesis."}
Abstract:
Recent advancements in the creation of artificial extra base pairs (unnatural base pairs, UBPs) are opening the door to a new research area, xenobiology, and genetic alphabet expansion technologies. UBPs that function as third base pairs in replication, transcription, and/or translation enable the site-specific incorporation of novel components into DNA, RNA, and proteins. Here, we describe the UBPs developed by three research teams and their application in PCR-based diagnostics, high-affinity DNA aptamer generation, site-specific labeling of RNAs, semi-synthetic organism creation, and unnatural amino- acid-containing protein synthesis.
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Publisher Copyright
Funding Info:
This research is supported by core funding from: Institute of Bioengineering and Nanotechnology
Grant Reference no. :

This research / project is supported by the A*STAR - Advanced Manufacturing and Engineering Programmatic Grant
Grant Reference no. : A18A9b0060

This research / project is supported by the National Research Foundation - Competitive Research Programme
Grant Reference no. : NRF-CRP17-2017- 07
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