Citation:
@Article{D0CS00457J,
author ="Kimoto, Michiko and Hirao, Ichiro",
title ="Genetic alphabet expansion technology by creating unnatural base pairs",
journal ="Chem. Soc. Rev.",
year ="2020",
volume ="49",
issue ="21",
pages ="7602-7626",
publisher ="The Royal Society of Chemistry",
doi ="10.1039/D0CS00457J",
url ="http://dx.doi.org/10.1039/D0CS00457J",
abstract ="Recent advancements in the creation of artificial extra base pairs (unnatural base pairs{,} UBPs) are opening the door to a new research area{,} xenobiology{,} and genetic alphabet expansion technologies. UBPs that function as third base pairs in replication{,} transcription{,} and/or translation enable the site-specific incorporation of novel components into DNA{,} RNA{,} and proteins. Here{,} we describe the UBPs developed by three research teams and their application in PCR-based diagnostics{,} high-affinity DNA aptamer generation{,} site-specific labeling of RNAs{,} semi-synthetic organism creation{,} and unnatural-amino-acid-containing protein synthesis."}