Y.-A. Lee, J.-J. Kim, J. Lee, J. H. J. Lee, S. Sahu, H.-Y. Kwon, S.-J. Park, S.-Y. Jang, J.-S. Lee, Z. Wang, W. L. Tam, B. Lim, N.-Y. Kang, Y.-T. Chang, Angew. Chem. Int. Ed. 2018, 57, 2851.
Abstract:
Tumor initiating cells (TICs) have been implicated in clinical relapse and metastasis of a variety of epithelial cancers, including lung cancer. While efforts toward the development of specific probes for TIC detection and targeting are ongoing, a universal TIC probe has yet to be developed. We report the first TIC‐specific fluorescent chemical probe, TiY, with identification of the molecular target as vimentin, a marker for epithelial‐to‐mesenchymal transition (EMT). TiY selectively stains TICs over differentiated tumor cells or normal cells, and facilitates the visualization and enrichment of functionally active TICs from patient tumors. At high concentration, TiY also shows anti‐TIC activity with low toxicity to non‐TICs. With the unexplored target vimentin, TiY shows potential as a first universal probe for TIC detection in different cancers.
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Funding Info:
This study was supported by an intramural funding from the A*STAR (Agency for Science, Technology and Research, Singapore) (core funding to W.L.T.) Biomedical Research Council, Institute for Basic Science (IBS) IBS-R007-A1, and the National Medical Research Council grant NMRC/TCR/016-NNI/2016. We thank SBIC-Nikon Imaging Centre for confocal microscopy facilities.
Description:
This is the peer reviewed version of the following article: Y.-A. Lee, J.-J. Kim, J. Lee, J. H. J. Lee, S. Sahu, H.-Y. Kwon, S.-J. Park, S.-Y. Jang, J.-S. Lee, Z. Wang, W. L. Tam, B. Lim, N.-Y. Kang, Y.-T. Chang, Angew. Chem. Int. Ed. 2018, 57, 2851., which has been published in final form at https://doi.org/10.1002/anie.201712920. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Use of Self-Archived Versions.