Genetic alphabet expansion biotechnology by creating unnatural base pairs

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Genetic alphabet expansion biotechnology by creating unnatural base pairs
Title:
Genetic alphabet expansion biotechnology by creating unnatural base pairs
Other Titles:
Current Opinion in Biotechnology
Keywords:
Publication Date:
16 October 2017
Citation:
Kyung Hyun Lee, Kiyofumi Hamashima, Michiko Kimoto, Ichiro Hirao, Genetic alphabet expansion biotechnology by creating unnatural base pairs, Current Opinion in Biotechnology, Volume 51, 2018, Pages 8-15, ISSN 0958-1669, https://doi.org/10.1016/j.copbio.2017.09.006.
Abstract:
Recent studies have made it possible to expand the genetic alphabet of DNA, which is originally composed of the four-letter alphabet with A–T and G–C pairs, by introducing an unnatural base pair (UBP). Several types of UBPs function as a third base pair in replication, transcription, and/or translation. Through the UBP formation, new components with different physicochemical properties from those of the natural ones can be introduced into nucleic acids and proteins site-specifically, providing their increased functionalities. Here, we describe the genetic alphabet expansion technology by focusing on three types of UBPs, which were recently applied to the creations of DNA aptamers that bind to proteins and cells and semi-synthetic organisms containing DNAs with a six-letter alphabet.
License type:
http://creativecommons.org/licenses/by-nc-nd/4.0/
Funding Info:
This work was supported by the Institute of Bioengineering and Nanotechnology (Biomedical Research Council, Agency for Science, Technology and Research, Singapore).
Description:
ISSN:
0958-1669
1879-0429
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