Large-scale image-based screening and profiling of cellular phenotypes

Large-scale image-based screening and profiling of cellular phenotypes
Title:
Large-scale image-based screening and profiling of cellular phenotypes
Other Titles:
Cytometry Part A
Keywords:
Publication Date:
19 July 2016
Citation:
Bougen‐Zhukov, N. , Loh, S. Y., Lee, H. K. and Loo, L. (2017), Large‐scale image‐based screening and profiling of cellular phenotypes. Cytometry, 91: 115-125. doi:10.1002/cyto.a.22909
Abstract:
Cellular phenotypes are observable characteristics of cells resulting from the interactions of intrinsic and extrinsic chemical or biochemical factors. Image-based phenotypic screens under large numbers of basal or perturbed conditions can be used to study the influences of these factors on cellular phenotypes. Hundreds to thousands of phenotypic descriptors can also be quantified from the images of cells under each of these experimental conditions. Therefore, huge amounts of data can be generated, and the analysis of these data has become a major bottleneck in large-scale phenotypic screens. Here, we review current experimental and computational methods for large-scale image-based phenotypic screens. Our focus is on phenotypic profiling, a computational procedure for constructing quantitative and compact representations of cellular phenotypes based on the images collected in these screens.
License type:
PublisherCopyrights
Funding Info:
1431AFG105
Description:
Full paper can be downloaded from the Publisher's URL provided.
ISSN:
1552-4922
1552-4930
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