Patthara Kongsuphol, Sunil K. Arya, Chee Chung Wong, Lee Joseph Polla, Mi Kyoung Park, Coiled-coil peptide based sensor for ultra-sensitive thrombin detection, Biosensors and Bioelectronics, Volume 55, 15 May 2014, Pages 26-31, ISSN 0956-5663, http://dx.doi.org/10.1016/j.bios.2013.11.070.
Abstract:
Comb structured gold microelectrode array (CSGMA) functionalized with self-assembled monolayer of
thiol terminated coiled-coilpeptide (CCP) linked together by the thrombin specific cleavage site (Leu–
Val–Pro–Arg–Gly–Ser) has been used to fabricate an ultrasensitive,disposable,electrochemical thrombin
biosensor. CCP with thiol at one end provides the ease of CSGMA functionalization and the presenceof
thrombin specific peptide in the middle of coiled-coilpeptide provides site for thrombin capture and
detection. CCP/CSGMA electrodes were characterized using label-freeelectrochemical impedance (EIS)
technique and exposed to solutions with different thrombin concentrations for its estimation.Results
reveal that CCP/CSGMA electrodes have a limit of detection (LOD) of10fg/ml(28fM) and are able to
detect catalytic activity of thrombin within 30 min time frame.CCP/CSGMA electrodes were found to be
selectiveagainstotherIgGanti-bodiessuchasDO1andHA.Thus,CCP/CSGMA electrodes provide high
specificity toward thrombin detection and mechanistic details of binding and cleavage process