Label free biosensor for sensitive human influenza virus hemagglutinin specific antibody detection using coiled-coil peptide modified microelectrode array based platform
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Label free biosensor for sensitive human influenza virus hemagglutinin specific antibody detection using coiled-coil peptide modified microelectrode array based platform
Label free biosensor for sensitive human influenza virus hemagglutinin specific antibody detection using coiled-coil peptide modified microelectrode array based platform
Sunil K. Arya, Patthara Kongsuphol, Chee Chung Wong, Lee Joseph Polla, Mi Kyoung Park, Label free biosensor for sensitive human influenza virus hemagglutinin specific antibody detection using coiled-coil peptide modified microelectrode array based platform, Sensors and Actuators B: Chemical, Volume 194, April 2014, Pages 127-133, ISSN 0925-4005, http://dx.doi.org/10.1016/j.snb.2013.12.066.
Abstract:
Coiled-coil peptide (CCP) modified microelectrode array with comb structure (MACS) has been utilized to
fabricate an electrochemical biosensor for antibody detection. Thiol terminated CCP has been employed
for self-assembling on MACS and peptide chains connecting amino acid sequence 98-106 (YPYDVPDYA)
of human Influenza virus hemagglutinin (HA) has been used as a molecular receptor for HA-antibody.
Electrochemical impedance (EIS) studies, cyclic voltammetry studies, atomic force microscopic imaging
and contact angle measurements were utilized to characterize self-assembly of CCP on MACS surface.
Further, EIS studies were used to investigate interaction between CCP and HA-antibody at different concentrations.
EIS measurements revealed that the specific interaction of HA-antibody with CCP gives rise
to a clear increase in the value of interfacial charge transfer resistance (Rct). A linear relationship between
Rct and the logarithm of HA-antibody concentration was found for the concentration range of 1 pg ml−1 to
100 ng ml−1, with a detection limit of 1 pg ml−1. Negligible change of Rct was observed by incubating the
sensor in solutions of other proteins such DO1, anti-GFAP, anti-IL6 and BSA, suggesting high selectivity of
descried immunosensor for HA-antibody. These results demonstrated that the CCP modified MACS based
platform can provide a versatile matrix for sensitive detection of desired target.