Qing Liu, Swee Yin Lim, Ross A. Soo, Mi Kyoung Park, Yong Shin, A rapid MZI-IDA sensor system for EGFR mutation testing in non-small cell lung cancer (NSCLC), Biosensors and Bioelectronics, Volume 74, 15 December 2015, Pages 865-871, ISSN 0956-5663, http://dx.doi.org/10.1016/j.bios.2015.07.055.
Abstract:
Epidermal growth factor receptor (EGFR) is a non-small-cell lung cancer biomarker,based on which
several near-patient-testing methods have been developed and applied to predict treatment response on
individual patients.Existing methods for detection of EGFR mutation are costly,labor-intensive and time-
consuming. In this paper,we report a novel EGFR mutation testing system,which is based on Mach–
Zehnder Interferometer (MZI) sensor and isothermal solid-phase DNA amplification (IDA) technique,
called MZI-IDA sensor system.The system can deliver results within 30 min and shows high sensitivity to
detect trace amounts of genomic DNA (o1 copy).In addition,the system is able to detect a L858R
mutation in a 99:1 mixture of wild-type and mutant cells.In a pilot clinical study,the system is com-
pared with conventional methods(PCR and direct sequencing) by using tissue biopsy samples from
NSCLC patients.The MZI-IDA sensor system is proved to be capable of fast and accurate detection of the
L858R mutation of EGFR gene in clinical samples.This may greatly help the clinicians develop an appropriate treatment plan.