Yu Chen, Seong-Jin Kim, Jinhong Guo, Yuejun Kang, Jaya P. Kausalya, Alicia Ghia Min Ong, Walter Hunziker, Jaehoon Chung, Portable Coulter counter with vertical through-holes for high-throughput applications, Sensors and Actuators B: Chemical, Volume 213, 5 July 2015, Pages 375-381, ISSN 0925-4005, http://dx.doi.org/10.1016/j.snb.2015.02.107. (http://www.sciencedirect.com/science/article/pii/S0925400515002920)
Abstract:
A high-throughput Coulter counter for point-of-care (POC) applications has been developed, which measures the concentration and size distribution of cells in suspension at a single cell resolution. Vertical channels (silicon-based through-holes), instead of conventional planar ones, were employed to achieve a higher density of sensing channels for a high-throughput operation. The crosstalk from the multiple sensing channels was minimized by potentiometric measurement based on a three-electrode configuration, decoupling the neighboring channels electrically. The through-hole array and potentiometric sensing increased throughput up to 8000 cells/s in the prototype (designed with four channels). To secure the sensitivity over a wide range of cell sizes in a limited setting (a portable device powered by a battery power,