The Helicobacter pylori Genome Project: insights into H. pylori population structure from analysis of a worldwide collection of complete genomes

The Helicobacter pylori Genome Project: insights into H. pylori population structure from analysis of a worldwide collection of complete genomes

Nature Communications

10.1038/s41467-023-43562-y

https://doi.org/10.1038/s41467-023-43562-y

Kaisa Thorell, Zilia Y. Muñoz-Ramírez, Difei Wang, Santiago Sandoval-Motta, Rajiv Boscolo Agostini, Silvia Ghirotto, Roberto C. Torres, , Judith Romero-Gallo, Uma Krishna, Richard M. Peek, M. Blanca Piazuelo, Naïma Raaf, Federico Bentolila, Hafeza Aftab, Junko Akada, Takashi Matsumoto, Freddy Haesebrouck, Rony P. Colanzi, Thais F. Bartelli, Diana Noronha Nunes, Adriane Pelosof, Claudia Zitron Sztokfisz, Emmanuel Dias-Neto, Paulo Pimentel Assumpção, Ivan Tishkov, Laure Brigitte Kouitcheu Mabeku, Karen J. Goodman, Janis Geary, Taylor J. Cromarty, Nancy L. Price, Douglas Quilty, Alejandro H. Corvalan, Carolina A. Serrano, Robinson Gonzalez, Arnoldo Riquelme, Apolinaria García-Cancino, Cristian Parra-Sepúlveda, Giuliano Bernal, Francisco Castillo, Alisa M. Goldstein, Nan Hu, Philip R. Taylor, Maria Mercedes Bravo, Alvaro Pazos, Luis E. Bravo, Keith T. Wilson, James G. Fox, Vanessa Ramírez-Mayorga, Silvia Molina-Castro, Sundry Durán-Bermúdez, Christian Campos-Núñez, Manuel Chaves-Cervantes, Evariste Tshibangu-Kabamba, Ghislain Disashi Tumba, Antoine Tshimpi-Wola, Patrick de Jesus Ngoma-Kisoko, Dieudonné Mumba Ngoyi, Modesto Cruz, Celso Hosking, José Jiménez Abreu, Christine Varon, Lucie Benejat, Ousman Secka, Alexander Link, Peter Malfertheiner, Michael Buenor Adinortey, Ansumana Sandy Bockarie, Cynthia Ayefoumi Adinortey, Eric Gyamerah Ofori, Dionyssios N. Sgouras, Beatriz Martinez-Gonzalez, Spyridon Michopoulos, Sotirios Georgopoulos, Elisa Hernandez, Braulio Volga Tacatic, Mynor Aguilar, Ricardo L. Dominguez, Douglas R. Morgan, Hjördís Harðardóttir, Anna Ingibjörg Gunnarsdóttir, Hallgrímur Guðjónsson, Jón Gunnlaugur Jónasson, Einar S. Björnsson, Mamatha Ballal, Vignesh Shetty, Muhammad Miftahussurur, Titong Sugihartono, Ricky Indra Alfaray, Langgeng Agung Waskito, Kartika Afrida Fauzia, Ari Fahrial Syam, Hasan Maulahela, Reza Malekzadeh, Masoud Sotoudeh, Avi Peretz, Maya Azrad, Avi On, Valli De Re, Stefania Zanussi, Renato Cannizzaro, Vincenzo Canzonieri, Takaya Shimura, Kengo Tokunaga, Takako Osaki, Shigeru Kamiya, Khaled Jadallah, Ismail Matalka, Nurbek Igissinov, Mariia Satarovna Moldobaeva, Attokurova Rakhat, Il Ju Choi, Jae Gyu Kim, Nayoung Kim, Minkyo Song, Mārcis Leja, Reinis Vangravs, Ģirts Šķenders, Dace Rudzīte, Aiga Rūdule, Aigars Vanags, Ilze Kikuste, Juozas Kupcinskas, Jurgita Skieceviciene, Laimas Jonaitis, Gediminas Kiudelis, Paulius Jonaitis, Vytautas Kiudelis, Greta Varkalaite, Jamuna Vadivelu, Mun Fai Loke, Kumutha Malar Vellasamy, Roberto Herrera-Goepfert, Juan Octavio Alonso-Larraga, Than Than Yee, Kyaw Htet, Takeshi Matsuhisa, Pradeep Krishna Shrestha, Shamshul Ansari, Olumide Abiodun, Christopher Jemilohun, Kolawole Oluseyi Akande, Oluwatosin Olu-Abiodun, Francis Ajang Magaji, Ayodele Omotoso, Chukwuemeka Chukwunwendu Osuagwu, Uchenna Okonkwo, Opeyemi O. Owoseni, Carlos Castaneda, Miluska Castillo, Billie Velapatino, Robert H. Gilman, Paweł Krzyżek, Grażyna Gościniak, Dorota Pawełka, Izabela Korona-Glowniak, Halina Cichoz-Lach, Monica Oleastro, Ceu Figueiredo, Jose C. Machado, Rui M. Ferreira, Dmitry S. Bordin, Maria A. Livzan, Vladislav V. Tsukanov, Patrick Tan, Khay Guan Yeoh, Feng Zhu, Reid Ally, Rainer Haas, Milagrosa Montes, María Fernández-Reyes, Esther Tamayo, Jacobo Lizasoain, Luis Bujanda, Sergio Lario, María José Ramírez-Lázaro, Xavier Calvet, Eduard Brunet-Mas, María José Domper-Arnal, Sandra García-Mateo, Daniel Abad-Baroja, Pedro Delgado-Guillena, Leticia Moreira, Josep Botargues, Isabel Pérez-Martínez, Eva Barreiro-Alonso, Virginia Flores, Javier P. Gisbert, Edurne Amorena Muro, Pedro Linares, Vicente Martin, Laura Alcoba, Tania Fleitas-Kanonnikoff, Hisham N. Altayeb, Lars Engstrand, Helena Enroth, Peter M. Keller, Karoline Wagner, Daniel Pohl, Yi-Chia Lee, Jyh-Ming Liou, Ming-Shiang Wu, Bekir Kocazeybek, Suat Sarıbas, İhsan Tasçı, Süleyman Demiryas, Nuray Kepil, Luis Quiel, Miguel Villagra, Morgan Norton, Deborah Johnson, Robert J. Huang, Joo Ha Hwang, Wendy Szymczak, Saranathan Rajagopalan, Emmanuel Asare, William R. Jacobs, Haejin In, Roni Bollag, Aileen Lopez, Edward J. Kruse, Joseph White, David Y. Graham, Charlotte Lane, Yang Gao, Patricia I. Fields, Benjamin D. Gold, Marcia Cruz-Correa, María González-Pons, Luz M. Rodriguez, Vo Phuoc Tuan, Ho Dang Quy Dung, Tran Thanh Binh, Tran Thi Huyen Trang, Vu Van Khien, Xiongfong Chen, Castle Raley, Bailey Kessing, Yongmei Zhao, Bao Tran, Andrés J. Gutiérrez-Escobar, Yunhu Wan, Belynda Hicks, Bin Zhu, Kai Yu, Bin Zhu, Meredith Yeager, Amy Hutchinson, Kedest Teshome, Kristie Jones, Wen Luo, Quentin Jehanne, Yukako Katsura, Patricio Gonzalez-Hormazabal, Xavier Didelot, Sam Sheppard, Eduardo Tarazona-Santos, Leonardo Mariño-Ramírez, John T. Loh, Steffen Backert, Michael Naumann, Christian C. Abnet, Annemieke Smet, Douglas E. Berg, Álvaro Chiner-Oms, Iñaki Comas, Francisco José Martínez-Martínez, Roxana Zamudio, Philippe Lehours, Francis Megraud, Koji Yahara, Martin J. Blaser, Tamas Vincze, Richard D. Morgan, Richard J. Roberts, Stephen J. Chanock, John P. Dekker, Javier Torres, Timothy L. Cover, Mehwish Noureen, Wolfgang Fischer, Filipa F. Vale, Joshua L. Cherry, Naoki Osada, Masaki Fukuyo, Masanori Arita, Yoshio Yamaoka, Ichizo Kobayashi, Ikuo Uchiyama, Daniel Falush, M. Constanza Camargo, Charles S. Rabkin

11 December 2023

Thorell, K., Muñoz-Ramírez, Z. Y., Wang, D., Sandoval-Motta, S., Boscolo Agostini, R., Ghirotto, S., Torres, R. C., Romero-Gallo, J., Krishna, U., Peek, R. M., Piazuelo, M. B., Raaf, N., Bentolila, F., Aftab, H., Akada, J., Matsumoto, T., Haesebrouck, F., Colanzi, R. P., … Rabkin, C. S. (2023). The Helicobacter pylori Genome Project: insights into H. pylori population structure from analysis of a worldwide collection of complete genomes. Nature Communications, 14(1). https://doi.org/10.1038/s41467-023-43562-y

Helicobacter pylori, a dominant member of the gastric microbiota, shares co-evolutionary history with humans. This has led to the development of genetically distinct H. pylori subpopulations associated with the geographic origin of the host and with differential gastric disease risk. Here, we provide insights into H. pylori population structure as a part of the Helicobacter pylori Genome Project (HpGP), a multi-disciplinary initiative aimed at elucidating H. pylori pathogenesis and identifying new therapeutic targets. We collected 1011 well-characterized clinical strains from 50 countries and generated high-quality genome sequences. We analysed core genome diversity and population structure of the HpGP dataset and 255 worldwide reference genomes to outline the ancestral contribution to Eurasian, African, and American populations. We found evidence of substantial contribution of population hpNorthAsia and subpopulation hspUral in Northern European H. pylori. The genomes of H. pylori isolated from northern and southern Indigenous Americans differed in that bacteria isolated in northern Indigenous communities were more similar to North Asian H. pylori while the southern had higher relatedness to hpEastAsia. Notably, we also found a highly clonal yet geographically dispersed North American subpopulation, which is negative for the cag pathogenicity island, and present in 7% of sequenced US genomes. We expect the HpGP dataset and the corresponding strains to become a major asset for H. pylori genomics.

Attribution 4.0 International (CC BY 4.0)

This research / project is supported by the National Research Foundation of Singapore, the Singapore Ministry of Health’s National Medical Research Council - Open Fund-Large Collaborative Grant
Grant Reference no. : MOH-OFLCG18May-0003

https://oar.a-star.edu.sg/communities-collections/articles/22606

2041-1723

Genome Institute of Singapore

https://doi.org/10.1038/s41467-023-43562-y