Landscape of mast cell populations across organs in mice and humans

Landscape of mast cell populations across organs in mice and humans

Journal of Experimental Medicine

10.1084/jem.20230570

https://doi.org/10.1084/jem.20230570

Marie Tauber, Lilian Basso, Jeremy Martin, Luciana Bostan, Marlene Magalhaes Pinto, Guilhem R. Thierry, Raïssa Houmadi, Nadine Serhan, Alexia Loste, Camille Bleriot, Jasper B.J. Kamphuis, Mirjana Grujic, Lena Kjellén, Gunnar Pejler, Carle Paul, Xinzhong Dong, Stephen J. Galli, Laurent L. Reber, Florent Ginhoux, Marc Bajénoff, Rebecca Gentek, Nicolas Gaudenzio

18 July 2023

Tauber, M., Basso, L., Martin, J., Bostan, L., Pinto, M. M., Thierry, G. R., Houmadi, R., Serhan, N., Loste, A., Blériot, C., Kamphuis, J. B. J., Grujic, M., Kjellén, L., Pejler, G., Paul, C., Dong, X., Galli, S. J., Reber, L. L., Ginhoux, F., … Gaudenzio, N. (2023). Landscape of mast cell populations across organs in mice and humans. Journal of Experimental Medicine, 220(10). https://doi.org/10.1084/jem.20230570

Mast cells (MCs) are tissue-resident immune cells that exhibit homeostatic and neuron-associated functions. Here, we combined whole-tissue imaging and single-cell RNA sequencing datasets to generate a pan-organ analysis of MCs in mice and humans at steady state. In mice, we identify two mutually exclusive MC populations, MrgprB2+ connective tissue–type MCs and MrgprB2neg mucosal-type MCs, with specific transcriptomic core signatures. While MrgprB2+ MCs develop in utero independently of the bone marrow, MrgprB2neg MCs develop after birth and are renewed by bone marrow progenitors. In humans, we unbiasedly identify six MC clusters/states (MC1–6) distributed across 12 organs with different transcriptomic core signatures. MC1 are preferentially enriched in the skin and lungs, MC2, MC3, and MC4 in the skin and bladder, MC5 in the lymph node and vasculature, and MC6 in the trachea and lungs. This comprehensive analysis offers valuable insights into the natural diversity of MC subtypes in both mice and humans.

Attribution 4.0 International (CC BY 4.0)

This work was supported by National Institutes of Health/National Institute of Allergy and Infectious Diseases R01AI132494 (to S.J. Galli), the Agence Nationale pour la Recherche, and the European Research Council (ERC-2018-STG #802041; to N. Gaudenzio).

©2023 Tauber et al. This article is available under a Creative Commons License (Attribution 4.0 International, as described at https://creativecommons.org/licenses/by/4.0/).

https://oar.a-star.edu.sg/communities-collections/articles/22544

0022-1007
1540-9538

Singapore Immunology Network

https://doi.org/10.1084/jem.20230570