MafB-restricted local monocyte proliferation precedes lung interstitial macrophage differentiation

MafB-restricted local monocyte proliferation precedes lung interstitial macrophage differentiation

Nature Immunology

10.1038/s41590-023-01468-3

https://doi.org/10.1038/s41590-023-01468-3

Domien Vanneste, Qiang Bai, Shakir Hasan, Wen Peng, Dimitri Pirottin, Joey Schyns, Pauline Maréchal, Cecilia Ruscitti, Margot Meunier, Zhaoyuan Liu, Céline Legrand, Laurence Fievez, Florent Ginhoux, Coraline Radermecker, Fabrice Bureau, Thomas Marichal

16 March 2023

Vanneste, D., Bai, Q., Hasan, S., Peng, W., Pirottin, D., Schyns, J., Maréchal, P., Ruscitti, C., Meunier, M., Liu, Z., Legrand, C., Fievez, L., Ginhoux, F., Radermecker, C., Bureau, F., & Marichal, T. (2023). MafB-restricted local monocyte proliferation precedes lung interstitial macrophage differentiation. Nature Immunology, 24(5), 827–840. https://doi.org/10.1038/s41590-023-01468-3

AbstractResident tissue macrophages (RTMs) are differentiated immune cells that populate distinct niches and exert important tissue-supportive functions. RTM maintenance is thought to rely either on differentiation from monocytes or on RTM self-renewal. Here, we used a mouse model of inducible lung interstitial macrophage (IM) niche depletion and refilling to investigate the development of IMs in vivo. Using time-course single-cell RNA-sequencing analyses, bone marrow chimeras and gene targeting, we found that engrafted Ly6C+ classical monocytes proliferated locally in a Csf1 receptor-dependent manner before differentiating into IMs. The transition from monocyte proliferation toward IM subset specification was controlled by the transcription factor MafB, while c-Maf specifically regulated the identity of the CD206+ IM subset. Our data provide evidence that, in the mononuclear phagocyte system, the ability to proliferate is not merely restricted to myeloid progenitor cells and mature RTMs but is also a tightly regulated capability of monocytes developing into RTMs in vivo.

Attribution 4.0 International (CC BY 4.0)

Fares, I. Sbai and A. Lio for their excellent administrative support. T.M. acknowledges support from the F.R.S-FNRS (Incentive Grant for Scientific Research), from the FRFS-Welbio and from the Acteria Foundation. T.M. is supported by a Research Project Grant of the F.R.S.-FNRS, by a FRFS-Welbio Advanced Grant (WELBIO-CR-2022A-10), by an ERC Starting Grant (ERC StG 2018 IM-ID: 801823), by the Baillet Latour Fund, by an ‘Action de Recherche Concertée de la Fédération Wallonie-Bruxelles de Belgique’ and by the Léon Fredericq Foundation; F.B. acknowledges support from the FRFS-Welbio and is supported by an Excellence of Science (EOS) program from the F.R.S.-FNRS and FWO; S.H. received a postdoctoral fellowship from Wallonie-Bruxelles International (WBI); D.V. and C. Ruscitti are research fellows of the F.R.S.-FNRS; W.P. is supported by a fellowship of the China Scholarship Council (CSC); M.M. is a research fellow of the FRIA – FNRS. C. Radermecker is a ‘Chargé de Recherches’ of the F.R.S.-FNRS.

https://oar.a-star.edu.sg/communities-collections/articles/22543

1529-2908
1529-2916

Singapore Immunology Network

https://doi.org/10.1038/s41590-023-01468-3