Moeglen-Paget, B., Perumal, J., Vedraine, S., Humbert, G., Olivo, M., & Dinish, U. S. (2025). Plasmonic-enhanced fluorescence measurements within an optofluidic photonic crystal fiber. APL Photonics, 10(5). https://doi.org/10.1063/5.0259030
Abstract:
We report the optimization of plasmonic-enhanced fluorescence (PEF) within an optofluidic photonic crystal fiber (PCF) for biosensing applications. This novel approach integrates silica-coated gold nanoparticles inside the inner walls of the PCF to enhance the signal of Cyanine 5 dye by associating the plasmonic enhancement of the fluorescence and the increased interaction length between the evanescent light and the analyte. This integration offers a robust solution for building a highly -sensitive detection platform. The thickness of the silica shell was optimized for maximizing the fluorescence enhancement while preventing fluorescence quenching effects. This study also investigates how the enhancement factor varies with different dye concentrations, resulting in a 150-fold enhancement under optimized conditions, demonstrating the system’s sensitivity and versatility. As a proof-of-concept model application, we have also demonstrated the detection of micro-RNA tagged with a fluorophore using a complementary DNA anchored within the PCF. We envision that the successful demonstration of PEF within a PCF probe could potentially translate into a highly sensitive optofluidic platform for biomarker detection.
License type:
Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0)
Funding Info:
This research was supported by ANR (Agence Nationale de la Recherche) and the NRF (National Research Foundation)