Teo, V. X., Bi, R., Lee, S. H., Chan, J., Thng, S. T. G., Olivo, M., & Dinish, U. S. (2025). Non‐Invasive Depth Profiling of Base Cosmetic Formulations in the Skin Using Handheld Confocal Raman Spectroscopy. Journal of Biophotonics. Portico. https://doi.org/10.1002/jbio.202400423
Abstract:
Ceramide‐based and aqueous‐based moisturizers have distinct absorption and retention characteristics in skin. Conventional methods like Transepidermal Water Loss (TEWL) and Corneometry are limited by environmental factors, and no current in vivo method measures ceramide content in skin. This research explores the use of a handheld Confocal Raman Spectroscopy (CRS) system to non‐invasively analyze the absorption kinetics of these moisturizers. Measurements were taken on 40 subjects, including healthy individuals (n = 20) and those with atopic dermatitis (n = 20). Results showed that ceramide‐based creams retained ceramide in the stratum corneum for longer periods, particularly 24 h post‐application, compared to aqueous‐based creams. Validation against Liquid Chromatography‐Mass Spectrometry (LCMS) for ceramide NP in four healthy subjects showed a strong correlation (r = 0.96). These findings suggest that handheld CRS can improve the evaluation of skincare formulations, advancing the development of personalized skincare solutions.
License type:
Publisher Copyright
Funding Info:
This research / project is supported by the Agency for Science, Technology and Research - Industry Alignment Fund - Pre-Positioning
Grant Reference no. : H19H6a0025
This research / project is supported by the Agency for Science, Technology and Research - Intramural Funding
Grant Reference no. :
This research / project is supported by the Agency for Science, Technology and Research - Central Research Fund (ATR) 2021
Grant Reference no. :
This research / project is supported by the National Health Innovation Centre Singapore (NHIC), Agency for Science, Technology and Research - NA
Grant Reference no. : CMTi-NHIC/19006
Description:
This is the peer reviewed version of the following article: Teo, V. X., Bi, R., Lee, S. H., Chan, J., Thng, S. T. G., Olivo, M., & Dinish, U. S. (2025). Non‐Invasive Depth Profiling of Base Cosmetic Formulations in the Skin Using Handheld Confocal Raman Spectroscopy. Journal of Biophotonics. Portico, which has been published in final form at https://doi.org/10.1002/jbio.202400423. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Use of Self-Archived Versions. This article may not be enhanced, enriched or otherwise transformed into a derivative work, without express permission from Wiley or by statutory rights under applicable legislation. Copyright notices must not be removed, obscured or modified. The article must be linked to Wiley’s version of record on Wiley Online Library and any embedding, framing or otherwise making available the article or pages thereof by third parties from platforms, services and websites other than Wiley Online Library must be prohibited.