Moeglen-Paget, B., Perumal, J., Humbert, G., Olivo, M., & Dinish, U. S. (2024). Optofluidic photonic crystal fiber platform for sensitive and reliable fluorescence based biosensing. Biomedical Optics Express, 15(7), 4281. https://doi.org/10.1364/boe.527248
Abstract:
Biosensing plays a pivotal role in various scientific domains, offering significant contributions to medical diagnostics, environmental monitoring, and biotechnology. Fluorescence biosensing relies on the fluorescence emission from labelled biomolecules to enable sensitive and selective identification and quantification of specific biological targets in various samples. Photonic crystal fibers (PCFs) have led to the development of optofluidic fibers enabling efficient light-liquid interaction within small liquid volume. Herein, we present the development of a user-friendly optofluidic-fiber platform with simple hardware requirements for sensitive and reliable fluorescence biosensing with high measurement repeatability. We demonstrate a sensitivity improvement of the fluorescence emission up to 17 times compared to standard cuvette measurement, with a limit of detection of Cy5 fluorophore as low as 100 pM. The improvement in measurement repeatability is exploited for detecting haptoglobin protein, a relevant biomarker to diagnose several diseases, by using commercially available Cy5 labelled antibodies. The study aims to showcase an optofluidic platform leveraging the benefits provided by optofluidic fibers, which encompass easy light injection, robustness, and high sensitivity.
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Attribution 4.0 International (CC BY 4.0)
Funding Info:
The authors acknowledge Institutional funding support from the National Research Agency
under the Investments for the future program with the reference ANR-18-EURE-0017 TACTIC, the project “FibOsome” funded by Nouvelle Aquitaine. This work was conducted within the frame of the International Research Project “FiberMed” from CNRS, A*STAR and Univ. Limoges.
This research / project is supported by the Agence Nationale de la Recherche (ANR), France - NA
Grant Reference no. : ANR-21-CE42-0027-01
This research / project is supported by the National Research Foundation Singapore, Agence Nationale de la Recherche (ANR), France - National Research Foundation - Agence Nationale de la Recherche Joint Bilateral Grant
Grant Reference no. : NRF2021-NRF-ANR002 FUNSENS
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Published by Optica Publishing Group under the terms of the Creative Commons Attribution 4.0 License. Further distribution of this work must maintain attribution to the author(s) and the published article’s title, journal citation, and DOI