Go, M. K., Zhu, T., Lim, K. J. H., Hartono, Y. D., Xue, B., Fan, H., & Yew, W. S. (2023). Cannabinoid Biosynthesis Using Noncanonical Cannabinoid Synthases. International Journal of Molecular Sciences, 24(2), 1259. https://doi.org/10.3390/ijms24021259
Abstract:
We report enzymes from the berberine bridge enzyme (BBE) superfamily that catalyze the oxidative cyclization of the monoterpene moiety in cannabigerolic acid (CBGA) to form cannabielsoin (CBE). The enzymes are from a variety of organisms and are previously uncharacterized. Out of 232 homologues chosen from the enzyme superfamily, four orthologues were shown to accept CBGA as a substrate and catalyze the biosynthesis of CBE. The four enzymes discovered in this study were recombinantly expressed and purified in Pichia pastoris. These enzymes are the first report of heterologous expression of BBEs that did not originate from the Cannabis plant that catalyze the production of cannabinoids using CBGA as substrate. This study details a new avenue for discovering and producing natural and unnatural cannabinoids.
License type:
Attribution 4.0 International (CC BY 4.0)
Funding Info:
This research / project is supported by the A*STAR - Industry Alignment Fund - Industry Collaboration Project (IAF-ICP)
Grant Reference no. : 12001E0068
This research / project is supported by the National Research Foundation (NRF) - Synthetic Biology Research and Development Program Grant
Grant Reference no. : SBP-P3