Oksuz, O., & Tee, W.-W. (2016). Probing Chromatin Modifications in Response to ERK Signaling. ERK Signaling, 289–301. doi:10.1007/978-1-4939-6424-6_22
Abstract:
Chromatin immunoprecipitation (ChIP) is a technique used to determine the association of proteins or histone modifications with chromatin regions in living cells or tissues, and is used extensively in the chromatin biology field to study transcriptional and epigenetic mechanisms. Increasing evidence points to an epigenetic coordination of signaling cascades, such as ERK, that regulate key processes in development and disease, revealing novel principles of gene regulation. Here we describe a detailed protocol for performing chromatin immunoprecipitation followed by qPCR (ChIP-qPCR) for probing histone modifications regulated by ERK signaling in mouse ESCs.
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Funding Info:
This research / project is supported by the Singapore National Research Foundation - Singapore National Research Foundation Fellowship
Grant Reference no. : None mentioned
This research is supported by core funding from: Biomedical Research Council, Agency for Science, Technology and Research
Grant Reference no. : N.A.