VAMP3/Syb and YKT6 are required for the fusion of constitutive secretory carriers with the plasma membrane

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VAMP3/Syb and YKT6 are required for the fusion of constitutive secretory carriers with the plasma membrane
Title:
VAMP3/Syb and YKT6 are required for the fusion of constitutive secretory carriers with the plasma membrane
Other Titles:
PLOS Genetics
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Publication Date:
12 April 2017
Citation:
Gordon DE, Chia J, Jayawardena K, Antrobus R, Bard F, Peden AA (2017) VAMP3/Syb and YKT6 are required for the fusion of constitutive secretory carriers with the plasma membrane. PLoS Genet 13(4): e1006698. https://doi.org/10.1371/journal.pgen.1006698
Abstract:
The cellular machinery required for the fusion of constitutive secretory vesicles with the plasma membrane in metazoans remains poorly defined. To address this problem we have developed a powerful, quantitative assay for measuring secretion and used it in combination with combinatorial gene depletion studies in Drosophila cells. This has allowed us to identify at least three SNARE complexes mediating Golgi to PM transport (STX1, SNAP24/29 and Syb; STX1, SNAP24/29 and YKT6; STX4, SNAP24 and Syb). RNAi mediated depletion of YKT6 and VAMP3 in mammalian cells also blocks constitutive secretion suggesting that YKT6 has an evolutionarily conserved role in this process. The unexpected role of YKT6 in plasma membrane fusion may in part explain why RNAi and gene disruption studies have failed to produce the expected phenotypes in higher eukaryotes.
License type:
http://creativecommons.org/licenses/by/4.0/
Funding Info:
AAP was funded by a MRC Career Development Award (MRC G120/952) and BBSRC grant (BB/L002841/1). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. Funding acquisition: AAP.
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ISSN:
1553-7390
1553-7404
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