Inflexibility of the Plasma miRNA Response Following a High-Carbohydrate Meal in Overweight Insulin-Resistant Women

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Inflexibility of the Plasma miRNA Response Following a High-Carbohydrate Meal in Overweight Insulin-Resistant Women
Title:
Inflexibility of the Plasma miRNA Response Following a High-Carbohydrate Meal in Overweight Insulin-Resistant Women
Journal Title:
Genes & Nutrition
Publication Date:
04 February 2020
Citation:
Ramzan, F., D’Souza, R.F., Durainayagam, B.R. et al. Inflexibility of the plasma miRNA response following a high-carbohydrate meal in overweight insulin-resistant women. Genes Nutr 15, 2 (2020). https://doi.org/10.1186/s12263-020-0660-8
Abstract:
Context: Metabolic inflexibility is a characteristic of insulin resistance, limiting the ability to transiently regulate oxidative metabolism and gene expression in response to nutrient availability. Little is known of the flexibility of post-transcriptional regulation, including circulatory miRNAs (c-miRNAs). Design: The abundances of targeted c-miRNAs, with reported functions in metabolic regulation, were analysed in response to a high-carbohydrate meal in healthy weight insulin-sensitive (IS) and overweight insulin-resistant (IR) women. Participants: Age-matched healthy weight IS (n = 20, BMI = 24.3 ± 0.70) and overweight IR (n = 20, BMI = 28.6 ± 0.67) women. Methods: An abundance of c-miRNAs was quantified prior to and following a high-carbohydrate breakfast meal (2500 kJ; 50% carbohydrate, 20% fat and 27% protein). Target genes of the differentially regulated c-miRNA were measured in RNA extracted from circulatory peripheral blood mononuclear cells (PBMCs). Results: In healthy weight IS women, both miR-15a-5p (p = 0.03) and miR-17-5p (p < 0.01) levels were halved at 4 h post-meal. These miRNA remained unaltered following the same meal in the overweight IR women. Furthermore, amongst genes targeted by these miRNA, CPT1A (p = 0.01) and IL8 (p = 0.03) had also reduced expression 4 h post-meal only in the healthy weight IS women. Conclusions: The study findings provide preliminary evidence for a possible extension of metabolic inflexibility to include c-miRNAs.
License type:
http://creativecommons.org/licenses/by/4.0/
Funding Info:
AgResearch Limited through the Strategic Science Investment Fund (nutritional strategies for an ageing population, contracts A19079 and A21246) supported this study
Description:
ISSN:
1555-8932
1865-3499
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