Ramzan, F., D’Souza, R.F., Durainayagam, B.R. et al. Inflexibility of the plasma miRNA response following a high-carbohydrate meal in overweight insulin-resistant women. Genes Nutr 15, 2 (2020). https://doi.org/10.1186/s12263-020-0660-8
Abstract:
Context:
Metabolic inflexibility is a characteristic of insulin resistance, limiting the ability to transiently regulate oxidative metabolism and gene expression in response to nutrient availability. Little is known of the flexibility of post-transcriptional regulation, including circulatory miRNAs (c-miRNAs).
Design:
The abundances of targeted c-miRNAs, with reported functions in metabolic regulation, were analysed in response to a high-carbohydrate meal in healthy weight insulin-sensitive (IS) and overweight insulin-resistant (IR) women.
Participants:
Age-matched healthy weight IS (n = 20, BMI = 24.3 ± 0.70) and overweight IR (n = 20, BMI = 28.6 ± 0.67) women.
Methods:
An abundance of c-miRNAs was quantified prior to and following a high-carbohydrate breakfast meal (2500 kJ; 50% carbohydrate, 20% fat and 27% protein). Target genes of the differentially regulated c-miRNA were measured in RNA extracted from circulatory peripheral blood mononuclear cells (PBMCs).
Results:
In healthy weight IS women, both miR-15a-5p (p = 0.03) and miR-17-5p (p
License type:
http://creativecommons.org/licenses/by/4.0/
Funding Info:
AgResearch Limited through the Strategic Science Investment Fund (nutritional strategies for an ageing population, contracts A19079 and A21246) supported this study