Microfluidic platform for negative enrichment of circulating tumor cells

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Microfluidic platform for negative enrichment of circulating tumor cells
Title:
Microfluidic platform for negative enrichment of circulating tumor cells
Other Titles:
Biomedical Microdevices
Keywords:
Publication Date:
27 March 2014
Citation:
Abstract:
Negative enrichment is the preferred approach for tumor cell isolation as it does not rely on bio marker expression. However, size-based negative enrichment methods suffer from well-known recovery/purity trade-off. Non-size based methods have a number of processing steps that lead to compounded cell loss due to extensive sample processing and handling which result in a low recovery efficiency. We present a method that performs negative enrichment in two steps from2 ml of whole blood in a total assay processing time of 60 min. This negative enrichment method employs upstream immunomagnetic depletion to deplete CD45-positive WBCs followed by a microfabricated filter membrane to perform chemical-free RBC depletion and target cells isolation. Experiments of spiking two cell lines, MCF-7 and NCIH1975, in the whole blood show an average of >90 % cell recovery over a range of spiked cell numbers. We also successfully recovered circulating tumor cells from 15 cancer patient samples.
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PublisherCopyrights
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ISSN:
1387-2176
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