Simões, E. A. F., Patel, C., Sung, W.-K., Lee, C. W. H., Loh, K. H. et al. Pathogen chip for respiratory tract infections. Journal of Clinical Microbiology 51, 945–953 (2013).
Abstract:
Determining the viral etiology of respiratory tract infections (RTI) has been limited for the most part to specific primer PCR-based methods due to their increased sensitivity and specificity compared to other methods, such as tissue culture. However, specific primer approaches have limited the ability to fully understand the diversity of infecting pathogens. A pathogen chip system (PathChip), developed at the Genome Institute of Singapore (GIS), using a random-tagged PCR coupled to a chip with over 170,000 probes, has the potential to recognize all known human viral pathogens. We tested 290 nasal wash specimens from Filipino children