Functional and Immunologic Mapping of Domains of the Reticulocyte-Binding Protein <i>Plasmodium vivax</i> PvRBP2a

Functional and Immunologic Mapping of Domains of the Reticulocyte-Binding Protein <i>Plasmodium vivax</i> PvRBP2a

The Journal of Infectious Diseases

10.1093/infdis/jiae111

http://dx.doi.org/10.1093/infdis/jiae111

Matthew Zirui Tay, Weiyi Tang, Wenn-Chyau Lee, Alice Soh Meoy Ong, Wisna Novera, Benoit Malleret, Guillaume Carissimo, Ann-Marie Chacko, Abbas El-Sahili, Julien Lescar, Yiping Fan, Rose M McGready, Cindy S Chu, Jerry Kok Yen Chan, Lisa F P Ng, Bruce Russell, François Nosten, Laurent Renia

05 March 2024

Tay, M. Z., Tang, W., Lee, W.-C., Ong, A. S. M., Novera, W., Malleret, B., Carissimo, G., Chacko, A.-M., El-Sahili, A., Lescar, J., Fan, Y., McGready, R. M., Chu, C. S., Chan, J. K. Y., Ng, L. F. P., Russell, B., Nosten, F., & Rénia, L. (2024). Functional and Immunologic Mapping of Domains of the Reticulocyte-Binding Protein Plasmodium vivax PvRBP2a. The Journal of Infectious Diseases. https://doi.org/10.1093/infdis/jiae111

We previously described a novel Plasmodium vivax invasion mechanism into human reticulocytes via the PvRBP2a-CD98 receptor-ligand pair. Using linear epitope mapping, we assessed the PvRBP2a epitopes involved in CD98 binding and recognized by antibodies from patients who were infected. We identified 2 epitope clusters mediating PvRBP2a-CD98 interaction. Cluster B (PvRBP2a431-448, TAALKEKGKLLANLYNKL) was the target of antibody responses in humans infected by P vivax. Peptides from each cluster were able to prevent live parasite invasion of human reticulocytes. These results provide new insights for development of a malaria blood-stage vaccine against P vivax.

Attribution 4.0 International (CC BY 4.0)

This research / project is supported by the The Wellcome Trust of Great Britain - Oxford Tropical Medicine Research Programme of Wellcome Trust–Mahidol University, to the Shoklo Malaria Research Unit
Grant Reference no. :

This research is supported by core funding from: Biomedical Research Council, A*STAR Infectious Diseases Labs
Grant Reference no. : NA

This research / project is supported by the Temasek Foundation - Temasek Foundation Infectious Diseases Programme for Surveillance and Diseases X Resilience
Grant Reference no. : NA

This research / project is supported by the National Medical Research Council - IRG Grant
Grant Reference no. : NMRC/OFIRG/0065/2018

This research / project is supported by the Ministry of Education, Singapore - Start-up University Grant
Grant Reference no. : 022388-00001

This research / project is supported by the National Medical Research Council - STaR Investigator Award
Grant Reference no. : NMRC STaR22jul-0004

This research / project is supported by the Agency for Science, Technology and Research - Health and Biomedical Sciences Industry Alignment Fund Pre-positioning
Grant Reference no. : H18/01/a0/018

This research / project is supported by the National Research Foundation Singapore - Immunomonitoring Service Platform - A*STAR’s Sign Flow Cytometry Platform
Grant Reference no. : NRF2017_SISFP09

This research / project is supported by the Marsden Grant Award - N/A
Grant Reference no. : RGUOO180301

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

https://oar.a-star.edu.sg/communities-collections/articles/20831

0022-1899
1537-6613

Infectious Disease Labs

https://academic.oup.com/jid/article-pdf/230/3/e737/59248641/jiae111.pdf