Optical biosensors utilising viral receptors ACE2 and ACE2 mimics

Page view(s)
7
Checked on Feb 04, 2025
Optical biosensors utilising viral receptors ACE2 and ACE2 mimics
Title:
Optical biosensors utilising viral receptors ACE2 and ACE2 mimics
Journal Title:
TrAC Trends in Analytical Chemistry
Publication Date:
04 March 2024
Citation:
Ow, S. Y., Sutarlie, L., Lim, S. W. Y., Salleh, N. A. B. M., Tanaka, Y., Tan, C. K. I., & Su, X. (2024). Optical biosensors utilising viral receptors ACE2 and ACE2 mimics. TrAC Trends in Analytical Chemistry, 173, 117630. https://doi.org/10.1016/j.trac.2024.117630
Abstract:
Numerous viral pathogens can cause pandemics, such as the COVID-19 pandemic caused by the SARS-CoV-2 virus. Rapid diagnosis is critical for timely treatment and for limiting the spread of the disease. Common viral infection starts from binding of the viral pathogens to the viral receptors on the host body, for example, SARS-CoV-2 infects cells by binding to human angiotensin converting enzyme 2 (ACE2) using the virus S protein and can be exploited for biosensor development. This review discusses optical biosensors that exploit ACE2 or ACE2 derived peptides (ACE2 mimics) as an affinity ligand for detecting SARS-CoV-2. In these ACE2- and ACE2-mimics-based optical biosensors, the target analytes can include whole virus (surrogate pseudoviruses and inactivated SARS-CoV-2 virus), spike proteins (S protein), or receptor binding domain (RBD) of the S protein. The optical transducer formats include lateral flow assays, colorimetric assays, fluorimetric assays, surface enhanced Raman spectroscopy, localised surface plasmon resonance etc. The sensor performances (such as limit of detection and turnover time) was compared among various optical biosensor formats. We highlight the advantages of using ACE2 and/or ACE2 mimics over common affinity ligands (such as antibodies) against future virus mutations. We also discussed the advantages of the smaller sized ACE2 mimics relative to ACE2 protein for achieving more versatile optical sensor designs involving nanomaterials and higher selectivity to SARS-CoV-2. In addition to SARS-CoV-2 detection, this review also discusses the expanded application of ACE2-based optical biosensors for the detection of non-viral targets, e.g., SARS-CoV-2 neutralising antibodies and anti-SARS-CoV-2 drugs.
License type:
Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0)
Funding Info:
This research / project is supported by the Ministry of Health, Singapore - Programme for Research in Epidemic Preparedness and Response (PREPARE),
Grant Reference no. : PREPARE-CS1-2022-003
Description:
Copyright © 2024 Elsevier B.V. All rights are reserved, including those for text and data mining, AI training, and similar technologies. This manuscript version is made available under the CC-BY-NC-ND 4.0 license.
ISSN:
0165-9936
Files uploaded:

File Size Format Action
trac-d-23-00601-revised-ms-30012024-accepted-manuscript-archive-ver.pdf 867.70 KB PDF Request a copy