Dual enzymes proteinosome with cascade activity for ultrasensitive glucose biosensing

Dual enzymes proteinosome with cascade activity for ultrasensitive glucose biosensing

Sensors and Actuators B: Chemical

10.1016/j.snb.2023.135067

https://doi.org/10.1016/j.snb.2023.135067

Tingting Wang, Xiaotong Fan, Yedong Ma, Zibiao Li, Chaobin He

30 November 2023

Wang, T., Fan, X., Ma, Y., Li, Z., & He, C. (2024). Dual enzymes proteinosome with cascade activity for ultrasensitive glucose biosensing. Sensors and Actuators B: Chemical, 402, 135067. https://doi.org/10.1016/j.snb.2023.135067

Catalytic cascades derived from the combination of multiple enzymes with efficient catalytic activity and high selectivity have attracted widespread attention in biosensing and biomedical applications. A key consideration in the development of cascade reaction systems is the combination of multiple enzymes to improve overall catalytic performance. Inspired by natural protein self-assembly, herein, proteinosomes formed by enzyme-surfactant complexes based on an interfacial assembly strategy are developed and they exhibit significant enhanced activity via cascade reaction of glucose oxidase (GOX) and horseradish peroxidase (HRP) that are located in proximity. The proteinosomes not only exhibit significantly elevated stability and reusability, but also possess enhanced cascade catalytic performance. These enhanced activities can be explained on the basis of the proximity effect of multiple enzymes within proteinosomes. Moreover, we employed highly efficient and reusability dual-enzyme-based proteinosomes to demonstrate the ultrasensitive detection of glucose, achieving a detection limit as low as 1.08 µM. Finally, the proteinosomes can be immobilized in a test strip for easy and fast glucose detection. Due to their ultra-sensitive detection limit, they can be used for non-invasive glucose detection. They can accurately assess glucose levels in saliva, yielding results highly congruent with those from commercially available assay kits. We further indicate the rapid capability of our system to detect glucose, as compared to commercial glucose kit. These finds demonstrate the potential of the test strip as a promising tool for daily glucose monitoring, and the facile approach presents an inventive solution for test strip development.

Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0)

This research is supported by the Agency for Science, Technology and Research (A*STAR) of Singapore

https://oar.a-star.edu.sg/communities-collections/articles/20746

402

Institute of Sustainability for Chemicals, Energy and Environment