Co‐Encapsulating Enzymes and Carbon Dots in Metal–Organic Frameworks for Highly Stable and Sensitive Touch‐Based Sweat Sensors

Page view(s)
6
Checked on Feb 10, 2025
Co‐Encapsulating Enzymes and Carbon Dots in Metal–Organic Frameworks for Highly Stable and Sensitive Touch‐Based Sweat Sensors
Title:
Co‐Encapsulating Enzymes and Carbon Dots in Metal–Organic Frameworks for Highly Stable and Sensitive Touch‐Based Sweat Sensors
Journal Title:
Advanced Functional Materials
Keywords:
Publication Date:
30 November 2023
Citation:
Zheng, X. T., Leoi, M. W. N., Yu, Y., Tan, S. C. L., Nadzri, N., Goh, W. P., Jiang, C., Ni, X. P., Wang, P., Zhao, M., & Yang, L. (2023). Co‐Encapsulating Enzymes and Carbon Dots in Metal–Organic Frameworks for Highly Stable and Sensitive Touch‐Based Sweat Sensors. Advanced Functional Materials, 34(10). Portico. https://doi.org/10.1002/adfm.202310121
Abstract:
Wearable metabolite sensors are often limited by easily denaturable enzymes that only allow short‐duration monitoring. Although encapsulating enzymes in metal–organic frameworks (MOFs) shows promise of long‐term enzyme protection, it is typically accompanied by significantly decreased activity due to increased diffusion barrier, steric hindrance for enzyme‐substrate binding, and poor enzyme‐electronic interface. Herein, the co‐encapsulation of enzymes and ultrasmall arginine‐derived carbon dots (Argdot) into a mesoporous Zeolitic Imidazolate Framework‐8 (mZIF‐8) matrix and the enhancement effect of Argdot on enzyme stability and activity, which consequently improves the electrochemical sensor's long‐term sensitivity are investigated. Specifically, the glucose oxidase (GOx)‐Argdot@mZIF‐8 nanocomposite consistently exhibits 40% higher electrochemical sensitivity compared to control GOx@mZIF‐8, an improvement similarly demonstrated with another model enzyme lactate oxidase (LOx). Furthermore, GOx‐Argdot@mZIF‐8 displays excellent stability, retaining 100% of initial sensitivity over 30 days of repeated testing at 37 °C. A touch‐based glucose sensor prototype is demonstrated as an excellent reusable sensor to monitor finger‐tip sweat glucose levels over one month at room temperature. This enzyme encapsulation strategy is not only useful for developing reusable sweat sensors with long‐term monitoring capability, but also promising to expand the industry use of enzymes under harsh conditions.
License type:
Publisher Copyright
Funding Info:
This research / project is supported by the Agency for Science, Technology and Research (A*STAR) - AME Programmatic Funds
Grant Reference no. : A18A1b0045

This research is supported by core funding from: A*STAR Central Research Fund (CRF)
Grant Reference no. : NA

This research / project is supported by the National Research Foundation - Singapore National Research Foundation Fellowships (NRFF)
Grant Reference no. : NA

This research / project is supported by the A*STAR - A*STAR Graduate Academy studentships
Grant Reference no. : NA

This research is supported by core funding from: Career Development Fund
Grant Reference no. : C210812027
Description:
This is the peer reviewed version of the following article: [Zheng, X. T., Leoi, M. W. N., Yu, Y., Tan, S. C. L., Nadzri, N., Goh, W. P., Jiang, C., Ni, X. P., Wang, P., Zhao, M., & Yang, L. (2023). Co‐Encapsulating Enzymes and Carbon Dots in Metal–Organic Frameworks for Highly Stable and Sensitive Touch‐Based Sweat Sensors. Advanced Functional Materials, 34(10). Portico. ], which has been published in final form at [https://doi.org/10.1002/adfm.202310121]. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Use of Self-Archived Versions. This article may not be enhanced, enriched or otherwise transformed into a derivative work, without express permission from Wiley or by statutory rights under applicable legislation. Copyright notices must not be removed, obscured or modified. The article must be linked to Wiley’s version of record on Wiley Online Library and any embedding, framing or otherwise making available the article or pages thereof by third parties from platforms, services and websites other than Wiley Online Library must be prohibited.
ISSN:
1616-301X
1616-3028
Files uploaded:

File Size Format Action
revised-manuscript.pdf 2.18 MB PDF Open