Pang, K. T., Yang, Y. S., Zhang, W., Ho, Y. S., Sormanni, P., Michaels, T. C. T., Walsh, I., & Chia, S. (2023). Understanding and controlling the molecular mechanisms of protein aggregation in mAb therapeutics. Biotechnology Advances, 67, 108192. https://doi.org/10.1016/j.biotechadv.2023.108192
Abstract:
In antibody development and manufacturing, protein aggregation is a common challenge that can lead to serious efficacy and safety issues. To mitigate this problem, it is important to investigate its molecular origins. This review discusses (1) our current molecular understanding and theoretical models of antibody aggregation, (2) how various stress conditions related to antibody upstream and downstream bioprocesses can trigger aggregation, and (3) current mitigation strategies employed towards inhibiting aggregation. We discuss the relevance of the aggregation phenomenon in the context of novel antibody modalities and highlight how in silico approaches can be exploited to mitigate it.
License type:
Attribution 4.0 International (CC BY 4.0)
Funding Info:
This research / project is supported by the Singapore Ministry of Health’s National Medical Research Council - Open Fund-Young Individual Research Grant (OF-YIRG)
Grant Reference no. : MOH-001132-00
This research / project is supported by the Agency for Science, Technology, and Research (A*STAR) - Start Up Fund
Grant Reference no. : NA
This research / project is supported by the Agency for Science, Technology, and Research (A*STAR) - Career Development Fund
Grant Reference no. : C210112057