Stem Cell Research Lab Resource: Stem Cell LineInduced pluripotent stem cell (iPSC) line MLi-003A derived from an individual with the maximum number of filaggrin (FLG) tandem repeats
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Stem Cell Research Lab Resource: Stem Cell LineInduced pluripotent stem cell (iPSC) line MLi-003A derived from an individual with the maximum number of filaggrin (FLG) tandem repeats
Stem Cell Research Lab Resource: Stem Cell LineInduced pluripotent stem cell (iPSC) line MLi-003A derived from an individual with the maximum number of filaggrin (FLG) tandem repeats
Khurana, P., Kolundzic, N., Rogar, M., Hobbs, C., Wong, X. F. C. C., Common, J. E. A., Ilic, D., & Liovic, M. (2020). Stem Cell Research Lab Resource: Stem Cell LineInduced pluripotent stem cell (iPSC) line MLi-003A derived from an individual with the maximum number of filaggrin (FLG) tandem repeats. Stem Cell Research, 45, 101827. https://doi.org/10.1016/j.scr.2020.101827
Abstract:
We have generated MLi003-A, a new induced pluripotent stem cell (iPSC) line derived from hair follicle kera-
tinocytes of a healthy male characterized with a maximum number of filaggrin tandem repeats, making this iPSC
line the best control for studies on skin barrier function. The characterization of the MLi003-A cell line consisted
of molecular karyotyping, high-throughput array-based sequencing composed of Fluidigm microfluidics tech-
nology and next-generation sequencing of the filaggrin alleles, and pluripotency and differentiation potentials
testing by immunofluorescence of associated markers both in vitro and in vivo. The MLi-003A line has been also
tested for ability to differentiate into keratinocytes.
License type:
Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0)
Funding Info:
This work was supported by an EMBO Short-Term Fellowship, the Republic of Slovenia Ministry of Education, Science and Sport, the EraCoSysMed (JTC-2 2017) “4D-HEALING” grant, and the Celsa Alliance grant.