Stem Cell Research Lab Resource: Stem Cell LineInduced pluripotent stem cell (iPSC) line MLi-003A derived from an individual with the maximum number of filaggrin (FLG) tandem repeats

Page view(s)
12
Checked on Aug 28, 2024
Stem Cell Research Lab Resource: Stem Cell LineInduced pluripotent stem cell (iPSC) line MLi-003A derived from an individual with the maximum number of filaggrin (FLG) tandem repeats
Title:
Stem Cell Research Lab Resource: Stem Cell LineInduced pluripotent stem cell (iPSC) line MLi-003A derived from an individual with the maximum number of filaggrin (FLG) tandem repeats
Journal Title:
Stem Cell Research
Keywords:
Publication Date:
24 April 2020
Citation:
Khurana, P., Kolundzic, N., Rogar, M., Hobbs, C., Wong, X. F. C. C., Common, J. E. A., Ilic, D., & Liovic, M. (2020). Stem Cell Research Lab Resource: Stem Cell LineInduced pluripotent stem cell (iPSC) line MLi-003A derived from an individual with the maximum number of filaggrin (FLG) tandem repeats. Stem Cell Research, 45, 101827. https://doi.org/10.1016/j.scr.2020.101827
Abstract:
We have generated MLi003-A, a new induced pluripotent stem cell (iPSC) line derived from hair follicle kera- tinocytes of a healthy male characterized with a maximum number of filaggrin tandem repeats, making this iPSC line the best control for studies on skin barrier function. The characterization of the MLi003-A cell line consisted of molecular karyotyping, high-throughput array-based sequencing composed of Fluidigm microfluidics tech- nology and next-generation sequencing of the filaggrin alleles, and pluripotency and differentiation potentials testing by immunofluorescence of associated markers both in vitro and in vivo. The MLi-003A line has been also tested for ability to differentiate into keratinocytes.
License type:
Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0)
Funding Info:
This work was supported by an EMBO Short-Term Fellowship, the Republic of Slovenia Ministry of Education, Science and Sport, the EraCoSysMed (JTC-2 2017) “4D-HEALING” grant, and the Celsa Alliance grant.
Description:
ISSN:
1873-5061
Collections:
Files uploaded:

File Size Format Action
1-s20-s1873506120301203-main.pdf 1.86 MB PDF Open