Enhancing stem cell therapy efficacy with functional lignin modified cerium-iron nanozyme through magnetic resonance imaging tracking and apoptosis protection in inflammatory environment

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Enhancing stem cell therapy efficacy with functional lignin modified cerium-iron nanozyme through magnetic resonance imaging tracking and apoptosis protection in inflammatory environment
Title:
Enhancing stem cell therapy efficacy with functional lignin modified cerium-iron nanozyme through magnetic resonance imaging tracking and apoptosis protection in inflammatory environment
Journal Title:
International Journal of Biological Macromolecules
Keywords:
Publication Date:
21 May 2024
Citation:
Huang, H., Zhang, L., Yew, P. Y. M., Sugiarto, S., Zhou, R., Kai, D., & Wang, Z. (2024). Enhancing stem cell therapy efficacy with functional lignin modified cerium-iron nanozyme through magnetic resonance imaging tracking and apoptosis protection in inflammatory environment. International Journal of Biological Macromolecules, 271, 132349. https://doi.org/10.1016/j.ijbiomac.2024.132349
Abstract:
Stem cell transplantation provides a promising approach for addressing inflammation and functional disorders. Nonetheless, the viability of these transplanted cells diminishes significantly within pathological environments, limiting their therapeutic potential. Moreover, the non-invasive tracking of these cells in vivo remains a considerable challenge, hampering the assessment of their therapeutic efficacy. Transition-metal oxide nanocrystals, known for their unique "enzyme-like" catalytic property and imaging capability, provide a new avenue for clinical application. In this study, the lignin as a biocompatible macromolecule was modified with poly (ethylene glycol) through chain-transfer polymerization, and then it was utilized to incorporate superparamagnetic iron oxide and cerium oxide nanocrystals creating a functional nanozyme. The iron oxide nanocrystals self-assembled into the hydrophobic core of nano system, while the in-situ mineralization of cerium oxide particles was carried out with the assistance of peripheral phenolic hydroxyl groups. The product, cerium‑iron core-shell nanozyme, enabled effective stem cells labeling through endocytosis and exhibited catalase and superoxide dismutase activities within the cells. As a result, it could scavenge highly destructive hydroxyl radicals and peroxyl radicals, shielding stem cells from apoptosis in inflammatory environment and maintaining their differentiation ability. Additionally, when these functionalized stem cells were administered to mice with acute inflammation, not only did they alleviate disease symptoms, but they also allowed for the visualization using T2-weighted magnetic resonance imaging. This innovative therapeutic approach provides a new strategy for combatting diseases.
License type:
Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0)
Funding Info:
This research / project is supported by the Agency of Science, Technology and Research (A*STAR) - RIE2025 MTC Individual Research Grants
Grant Reference no. : M22K2c0085

This research / project is supported by the National Medical Research Council (NMRC) - Clinician Scientist-Individual Research Grant
Grant Reference no. : MOH-001357-00

This research / project is supported by the Agency of Science, Technology and Research (A*STAR) - Urban and Green Technology Horizontal Technology Seed Fund
Grant Reference no. : C211718009

This research was supported by the Natural Science Foundation of the Guangdong Province, Guangdong Basic and Applied Basic Research Foundation (2021A1515011799 and 2023A1515010820). Science and Technology Project of Yantian District in Shenzhen City, Guangdong Province, China (YTWS20220206).
Description:
ISSN:
0141-8130
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