Sofeo, N., Toi, M. G., Ee, E. Q. G., Ng, J. Y., Busran, C. T., Lukito, B. R., Thong, A., Hermansen, C., Peterson, E. C., Glitsos, R., & Arumugam, P. (2024). Sustainable production of lipids from cocoa fatty acid distillate fermentation driven by adaptive evolution in Yarrowia lipolytica. Bioresource Technology, 394, 130302. https://doi.org/10.1016/j.biortech.2024.130302
Abstract:
Single cell oil production using oleaginous yeasts is a promising alternative to animal and plant-derived lipids. But substrate costs for microbial fermentation are a major bottleneck. Using side streams as alternative to substrates like glucose, for growing yeast, is a potential cost-effective solution. By combining a previously reported process of growing yeasts on a solid cocoa fatty acid distillate side stream with adaptive evolution techniques, the growth of oleaginous yeast Yarrowia lipolytica was improved by 2-fold. The lipid titre was also boosted by more than 3-fold. Using transcriptomics, key genes were identified that are possibly involved in tailoring of lipid composition, side stream utilisation and enhancement of lipid titres. Candidate genes were also identified that might enable efficient growth and utilization of fatty acids and triacylglycerides found in cocoa fatty acid distillate. In summary, this research has improved the understanding of side stream utilisation for lipid production in oleaginous yeast.
License type:
Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0)
Funding Info:
This research / project is supported by the The Good Food Institute (GFI) - GFI White Space Grant Program, RFP 2021
Grant Reference no. : NA
This research / project is supported by the Agency for Science, Technology, and Research (A*STAR), Republic of Singapore - 2021 Horizontal Technology Coordinating Office Seed Fund
Grant Reference no. : C211018002