3D Bioprinted Xanthan Hydrogels with Dual Antioxidant and Chondrogenic Functions for Post-traumatic Cartilage Regeneration

3D Bioprinted Xanthan Hydrogels with Dual Antioxidant and Chondrogenic Functions for Post-traumatic Cartilage Regeneration

ACS Biomaterials Science & Engineering

10.1021/acsbiomaterials.3c01636

http://dx.doi.org/10.1021/acsbiomaterials.3c01636

Yuting Chen, Yiguan Le, Junxu Yang, Yifeng Yang, Xianjing Feng, Jinhong Cai, Yifeng Shang, Sigit Sugiarto, Qingjun Wei, Dan Kai, Li Zheng, Jinmin Zhao

16 February 2024

Chen, Y., Le, Y., Yang, J., Yang, Y., Feng, X., Cai, J., Shang, Y., Sugiarto, S., Wei, Q., Kai, D., Zheng, L., & Zhao, J. (2024). 3D Bioprinted Xanthan Hydrogels with Dual Antioxidant and Chondrogenic Functions for Post-traumatic Cartilage Regeneration. ACS Biomaterials Science & Engineering, 10(3), 1661–1675. https://doi.org/10.1021/acsbiomaterials.3c01636

Intra-articular trauma typically initiates the overgeneration of reactive oxidative species (ROS), leading to post-traumatic osteoarthritis and cartilage degeneration. Xanthan gum (XG), a branched polysaccharide, has shown its potential in many biomedical fields, but some of its inherent properties, including undesirable viscosity and poor mechanical stability, limit its application in 3D printed scaffolds for cartilage regeneration. In this project, we developed 3D bioprinted XG hydrogels by modifying XG with methacrylic (MA) groups for post-traumatic cartilage therapy. Our results demonstrated that the chemical modification optimized the viscoelasticity of the bioink, improved printability, and enhanced the mechanical properties of the resulting scaffolds. The XG hydrogels also exhibit decent ROS scavenging capacities to protect stem cells from oxidative stress. Furthermore, XGMA(H) (5% MA substitution) exhibited superior chondrogenic potential in vitro and promoted cartilage regeneration in vivo. These dual-functional XGMA hydrogels may provide a new opportunity for cartilage tissue engineering.

Publisher Copyright

This research / project is supported by the ASTAR - MTC Individual Research Grants
Grant Reference no. : M22K2c0085

This work was supported by the Guangxi Science and Technology Base and Talent Special Project (Grant No. GuikeAD21075002), Natural Science Foundation of Guangxi (Grant No. 2020GXNSFAA159134), National Natural Science Foundation of China (Grant No. 82160430), Guangxi Science and Technology Major Project (Grant No. GuikeAA19254002), and Nanning Qingxiu District Science and Technology Major Special Project (Grant No. 2020013).

This document is the Accepted Manuscript version of a Published Work that appeared in final form in ACS Biomaterials Science & Engineering, copyright © American Chemical Society after peer review and technical editing by the publisher. To access the final edited and published work see doi.org/10.1021/acsbiomaterials.3c01636

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Institute of Materials Research and Engineering