Identification of a pharyngeal mucosal lymphoid organ in zebrafish and other teleosts: Tonsils in fish?

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Identification of a pharyngeal mucosal lymphoid organ in zebrafish and other teleosts: Tonsils in fish?
Title:
Identification of a pharyngeal mucosal lymphoid organ in zebrafish and other teleosts: Tonsils in fish?
Journal Title:
Science Advances
Publication Date:
01 November 2023
Citation:
Resseguier, J., Nguyen-Chi, M., Wohlmann, J., Rigaudeau, D., Salinas, I., Oehlers, S. H., Wiegertjes, G. F., Johansen, F.-E., Qiao, S.-W., Koppang, E. O., Verrier, B., Boudinot, P., & Griffiths, G. (2023). Identification of a pharyngeal mucosal lymphoid organ in zebrafish and other teleosts: Tonsils in fish? Science Advances, 9(44). https://doi.org/10.1126/sciadv.adj0101
Abstract:
The constant exposure of the fish branchial cavity to aquatic pathogens causes local mucosal immune responses to be extremely important for their survival. Here, we used a marker for T lymphocytes/natural killer (NK) cells (ZAP70) and advanced imaging techniques to investigate the lymphoid architecture of the zebrafish branchial cavity. We identified a sub-pharyngeal lymphoid organ, which we tentatively named “Nemausean lymphoid organ” (NELO). NELO is enriched in T/NK cells, plasma/B cells, and antigen-presenting cells embedded in a network of reticulated epithelial cells. The presence of activated T cells and lymphocyte proliferation, but not V(D)J recombination or hematopoiesis, suggests that NELO is a secondary lymphoid organ. In response to infection, NELO displays structural changes including the formation of T/NK cell clusters. NELO and gill lymphoid tissues form a cohesive unit within a large mucosal lymphoid network. Collectively, we reveal an unreported mucosal lymphoid organ reminiscent of mammalian tonsils that evolved in multiple teleost fish families.
License type:
Attribution 4.0 International (CC BY 4.0)
Funding Info:
This research is supported by core funding from: ID Labs
Grant Reference no. : Core

This work was supported by the Norwegian Research Council for funding (nos. 144642and329478). The Agence Nationale de la Recherche (France) (project ANR-21-CE35-0019) and by the ERANET project Nucnanofish (ANR-21-ICRD-0009). The U.S. Department of Agriculture/National Institute of Food and Agriculture grant 2019-05906.M.N.-C.was supported by the French Agence Nationale de la Recherche (ANR-19-CE15-0005-01,MacrophageDynamics).
Description:
ISSN:
2375-2548