Characterizing date seed polysaccharides: A comprehensive study on extraction, biological activities, prebiotic potential, gut microbiota modulation, and rheology using microwave-assisted deep eutectic solvent
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Characterizing date seed polysaccharides: A comprehensive study on extraction, biological activities, prebiotic potential, gut microbiota modulation, and rheology using microwave-assisted deep eutectic solvent
Characterizing date seed polysaccharides: A comprehensive study on extraction, biological activities, prebiotic potential, gut microbiota modulation, and rheology using microwave-assisted deep eutectic solvent
Jayasree Subhash, A., Babatunde Bamigbade, G., al-Ramadi, B., Kamal-Eldin, A., Gan, R.-Y., Senaka Ranadheera, C., & Ayyash, M. (2024). Characterizing date seed polysaccharides: A comprehensive study on extraction, biological activities, prebiotic potential, gut microbiota modulation, and rheology using microwave-assisted deep eutectic solvent. Food Chemistry, 444, 138618. https://doi.org/10.1016/j.foodchem.2024.138618
Abstract:
This study investigated the biological activities, prebiotic potentials, modulating gut microbiota, and rheological properties of polysaccharides derived from date seeds via microwave-assisted deep eutectic solvent systems. Averaged molecular weight (246.5 kDa) and a monosaccharide profile (galacturonic acid: glucose: mannose: fructose: galactose), classifying MPS as a heteropolysaccharide. MPS, at concentrations of 125–1000 µg/mL, demonstrates increasing free radical scavenging activities (DPPH, ABTS, MC, SOD, SORS, and LO), potent antioxidant potential (FRAP: 51.2–538.3 µg/mL; TAC: 28.3–683.4 µg/mL; RP: 18.5–171.2 µg/mL), and dose-dependent antimicrobial activity against common foodborne pathogens. Partially-purified MPS exhibits inhibition against α-glucosidase (79.6 %), α-amylase (85.1 %), and ACE (68.4 %), along with 80 % and 46 % inhibition against Caco-2 and MCF-7 cancer cells, respectively. Results indicate that MPS fosters the growth of beneficial fecal microbiota, including Proteobacteria, Firmicutes, and Actinobacteria, supporting microbes responsible for major SCFAs (acetic, propionic, and butyric acids) production, such as Ruminococcus and Blautia.
License type:
Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0)
Funding Info:
The authors are thankful to the United Arab Emirates University and Zayed Center for Health Sciences (UAEU) for funding this project (grant number # 12R105)